Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tracking changes in functionality and morphology of repopulated microglia in young and old mice

ABSTRACT: Tracking changes in functionality and morphology of repopulated microglia in young and old mice

PROVIDER: PRJNA1132223 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Tracking changes in functionality and morphology of repopulated microglia in young and old mice [RNA-Seq]

Project description:Tracking changes in functionality and morphology of repopulated microglia in young and old mice [RNA-Seq]

| PRJNA1132218 | ENA

Tracking changes in functionality and morphology of repopulated microglia in young and old mice

Project description:Microglia (MG) are resident myeloid cells in the central nervous system (CNS) that perform homeostatic or pathological functions. Microglia self-renewal is controlled by the macrophage colony-stimulating factor-1 (CSF-1). Inhibitors of CSF-1 receptor (i.a. BLZ-945) by blocking the CSF1R signaling deplete 99% of microglia in 21 days and after cessation of treatment microglia rapidly repopulate and restore normal density within 1 week in adult mice. The functions of these repopulated microglia are poorly known and they can vary according to age. To investigate the microglia repopulation process, we compared samples of repopulated microglia from young (3 months) and old (12 months) mice using single cell RNA sequencing (scRNA-seq).

2024-11-27 | GSE271560 | GEO

Tracking changes in functionality and morphology of repopulated microglia in young and old mice [RNA-Seq]

Project description:Microglia (MG) are resident myeloid cells in the central nervous system (CNS) that perform homeostatic or pathological functions. Microglia self-renewal is controlled by the macrophage colony-stimulating factor-1 (CSF-1). Inhibitors of CSF-1 receptor (i.a. BLZ-945) by blocking the CSF1R signaling deplete 99% of microglia in 21 days and after cessation of treatment microglia rapidly repopulate and restore normal density within 1 week in adult mice. The functions of these repopulated microglia are poorly known and they can vary according to age. To investigate the microglia repopulation process, we compared samples of repopulated microglia from old (18-22 months) mice against undepleted controls using RNA sequencing (RNA-seq).

2024-11-27 | GSE271559 | GEO

Neural stem cells of Parkinson’s disease patients exhibit aberrant mitochondrial morphology and functionality

Project description:Neural stem cells of Parkinson’s disease patients exhibit aberrant mitochondrial morphology and functionality

| PRJNA526151 | ENA

Neural stem cells of Parkinson’s disease patients exhibit aberrant mitochondrial morphology and functionality

Project description:Emerging evidence suggest that Parkinson's disease (PD), besides being an age-associated disorder, might also have a neurodevelopment component. Disruption of mitochondrial homeostasis has been highlighted as a crucial cofactor in its etiology. Here, we show that PD patient-specific human neuroepithelial stem cells (NESCs) carrying the LRRK2-G2019S mutation recapitulate key mitochondrial defects previously described only in differentiated dopaminergic neurons. By combining high-content imaging approaches, 3D image analysis, and functional mitochondrial readouts we show that LRRK2-G2019S mutation caused aberrations in mitochondrial morphology and functionality compared to isogenic controls. LRRK2-G2019S NESCs displayed an increased number of mitochondria compared to isogenic control lines. However, these mitochondria were more fragmented and exhibited decreased membrane potential. Coherently, the release of total and mitochondrial redox oxidative species increased in LRRK2-G2019S NESC compared to controls. Functional alterations in LRRK2-G2019S cultures were also accompanied by a reduced mitophagic clearance via lysosomes. These findings support the hypothesis that preceding mitochondrial developmental defects contribute to the manifestation of the PD pathology later in life.

2019-03-09 | GSE128040 | GEO

The RNA sequencing of microglia repopulated retina

Project description:The RNA sequencing of microglia repopulated retina

| PRJNA414904 | ENA

Single-cell RNA sequencing of repopulated microglia at day 3

Project description:To probe potential molecular mechanisms underlying the differences between wild-type and Hk2-cKO microglia during repopulation, we performed the single-cell RNA sequencing (scRNA-seq) of repopulated microglia at Day 3. Microglia were isolated by FACS as CD11b high CD45 low cells from normal brains and Hk2-cKO brains.

2021-12-17 | GSE188642 | GEO

Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2014

Project description:Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2014

| PRJNA509614 | ENA

Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2013

Project description:Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2013

| PRJNA509612 | ENA

Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2015

Project description:Longitudinal tracking of whole blood gene-expression in healthy young and old individuals - 2015

| PRJNA509613 | ENA