Methanosarcina spindle-shaped virus 1
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1132277 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Nitrosopumilus spindle-shaped viruses (NSV) of genome sequencing and assembly
| PRJNA516109 | ENA
Project description:Methanosarcina spherical virus Raw sequence reads
| PRJNA980962 | ENA
Project description:Bipolar spindle assembly and chromosome biorientation are prerequisites for chromosome segregation during cell division. The kinesin motor KIF11 (also widely known as Eg5) drives spindle bipolarization by sliding antiparallel microtubules bidirectionally, elongating a spherical spindle into a bipolar-shaped structure in acentrosomal oocytes. During meiosis I, this process stretches homologous chromosome pairs, establishing chromosome biorientation at the spindle equator. The quantitative requirement for KIF11 in acentrosomal spindle bipolarization and homologous chromosome biorientation remains unclear. Here, using a genetic strategy to modulate KIF11 expression levels, we show that Kif11 haploinsufficiency impairs spindle elongation, leading to the formation of a partially bipolarized spindle during meiosis I in mouse oocytes. While the partially bipolarized spindle allows chromosome stretching in the inner region of its equator, it fails to do so in the outer region, where merotelic kinetochore-microtubule attachments are favored to form. These findings demonstrate the necessity of biallelic functional Kif11 for bipolar spindle assembly in acentrosomal oocytes and reveal a spatially differential requirement for homologous chromosome biorientation within the spindle.
2025-07-01 | GSE284383 | GEO
Project description:Dual-RNAseq of Methanosarcina mazei infected with Methanosarcina spherical virus (MetSV) Transcriptome
| PRJNA883050 | ENA
Project description:The transition from meiotic divisions in the oocyte to mitotic divisions in the early embryo is a critical step in animal development. Despite negligible changes to cell size, shape, and content, following fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle that nucleates abundant astral microtubules at spindle poles. To identify underlying mechanisms, we applied a drug screening approach using eggs of the simple chordate Ciona robusta (sea squirt) and found that inhibition of Casein Kinase 2 (CK2) caused a shift from meiotic to mitotic-like spindle morphology with nucleation of robust astral microtubule arrays, an effect reproduced in Xenopus laevis (frog) egg extracts. In both species, a kinase assay revealed that CK2 activity decreases at fertilization. To identify substrates and downstream effectors, we assessed the global phosphoproteomic changes that accompany both the meiosis to mitosis transition and CK2 inhibition in Xenopus, which identified components of the Ran-GTP pathway as potential downstream targets. Inhibition of Ran-GTP-driven microtubule nucleation suppressed CK2-induced spindle morphology changes in both Xenopus and Ciona. These data support a model in which attenuation of CK2 activity at fertilization in chordates leads to activation of Ran-regulated spindle assembly factors that drive microtubule growth and the transition to mitotic spindle morphology.
2024-07-04 | MSV000095250 | MassIVE
Project description:Methanosarcina acetivorans Genome sequencing and assembly
| PRJNA477340 | ENA
Project description:Methanosarcina mazei Genome sequencing and assembly
| PRJNA557089 | ENA
Project description:Methanosarcina acetivorans Genome sequencing and assembly
| PRJNA859536 | ENA