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Total RNA-seq and long-read RNAseq (Nanopore) upon Son KD in mouse embryonic stem cells

ABSTRACT: Total RNA-seq and long-read RNAseq (Nanopore) upon Son KD in mouse embryonic stem cells

PROVIDER: PRJNA1132708 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29728200_1.fastq.gz	Fastqsanger.gz
	SRR29728201_1.fastq.gz	Fastqsanger.gz
	SRR29728201_2.fastq.gz	Fastqsanger.gz
	SRR29728202_1.fastq.gz	Fastqsanger.gz
	SRR29728202_2.fastq.gz	Fastqsanger.gz

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Total RNA-seq and long-read RNAseq (Nanopore) upon Son KD in mouse embryonic stem cells

Project description:Gene-expression noise can influence cell-fate choices across pathology and physiology. However, a crucial question persists: do regulatory proteins or pathways exist that control noise independently of mean expression levels? Resulting from a previous screen, the protein SON was identified as a potential noise regulator. We perform Son KD and utilize (1) total RNA sequencing (RNA-seq) to analyze differences in splicing efficiency of introns upon Son KD; and (2) Nanopore sequencing to analyze changes in isoform usage. These datasets correspond to the aforementioned total RNA-seq and long-read polyA+ cDNA Nanopore sequencing upon Son KD.

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Son KD in mouse embryonic stem cells

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Son KD in mouse embryonic stem cells

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