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Forest musk feces

ABSTRACT: Forest musk feces Raw sequence reads

PROVIDER: PRJNA1135790 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR32206242_1.fastq.gz	Fastqsanger.gz
	SRR32206242_2.fastq.gz	Fastqsanger.gz
	SRR32206243_1.fastq.gz	Fastqsanger.gz
	SRR32206243_2.fastq.gz	Fastqsanger.gz
	SRR32206245_1.fastq.gz	Fastqsanger.gz

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Project description:The Chinese forest musk deer (FMD; Moschus berezovskii) is an endangered artiodactyl mammal. Musk secreted by the musk gland of male FMD has extremely high economic and medicinal value. At present, little is known about the development of musk glands and the molecular mechanism of musk secretion. In the present research, using snRNA-seq and snATAC-seq association analysis performed on musk glands of forest musk deer, coupled with several bioinformatics analyses, the dynamic transcriptional cell atlas of musk gland development was revealed and the genes and transcription factors affecting musk secretion were determined. Based on uniform manifold approximation and projection (UMAP) analysis, we identified 12 cell types from musk glands, including two different acinar cells (clusters 0 and 10). In addition, the expression of core target genes and core transcription factors was verified by fluorescence in situ hybridization and immunohistochemistry. Combined with weighted gene co-expression network analysis (WGCNA), we obtained a deeper biological understanding of the relationship between core transcription factors, differentially expressed genes and musk secretion related pathways. This study lays a foundation for improving musk yield and meeting market demand. In the meantime, it also contributes to reducing the hunting and poaching of wild forest musk deer, protecting forest musk deer resources and maintaining ecological balance.

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