Project description:This SuperSeries is composed of the following subset Series: GSE21992: Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq GSE22001: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq GSE22002: Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling GSE22003: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling Refer to individual Series

2010-08-02 | E-GEOD-22004 | biostudies-arrayexpress

Wild-type cultured neutrophils versus miR-223 null cultured neutrophils

Project description:This array analysis is to study the regulation of target messages’ expression in in vitro cultured murine neutrophils versus miR-223 null neutrophils. Culture media was SILAC-IMDM for MS analysis.

2008-07-31 | GSE11973 | GEO

Mus musculus

Project description:4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils

| PRJNA114299 | ENA

Wild-type neutrophils and miR-223 null neutrophils

Project description:This array analysis is to study the regulation of target messages’ expression in murine neutrophils versus miR-223 null neutrophils.

2008-07-31 | GSE12001 | GEO

4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils

Project description:This array analysis is to study developmental time course of the regulation of target messages’ expression during culture of murine neutrophils versus miR-223 null neutrophils. Culture media was SILAC-IMDM for MS analysis.

2008-07-30 | GSE12003 | GEO

Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq

Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine ribosome footprints and mRNA abundance of mir-223 knockout cultured neutrophils, versus wild-type cultured neutrophils Supplementary processed data file linked below. mir223_summaryTable.txt: log2 fold changes (miR-223-knockout versus wild-type neutrophils).

2010-08-03 | E-GEOD-22001 | biostudies-arrayexpress

Mus musculus

Project description:miR-1-2 knockout versus wild-type hearts

| PRJNA98057 | ENA