Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bombyx mori UFBP1 regulates apoptosis and promotes BmNPV proliferation by affecting the expression of ER chaperone BIP

ABSTRACT: Bombyx mori UFBP1 regulates apoptosis and promotes BmNPV proliferation by affecting the expression of ER chaperone BIP

PROVIDER: PRJNA1138767 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29920263_1.fastq.gz	Fastqsanger.gz
	SRR29920263_2.fastq.gz	Fastqsanger.gz
	SRR29920264_1.fastq.gz	Fastqsanger.gz
	SRR29920264_2.fastq.gz	Fastqsanger.gz
	SRR29920265_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Bombyx mori UFBP1 regulates apoptosis and promotes BmNPV proliferation by affecting the expression of ER chaperone BIP

Project description:Ubiquitin-fold modifier 1 (UFM1) is attached to protein substrates through the sequential activity of an E1 (UBA5)-E2 (UBC1)-E3 (UBL1) cascade. UFBP1 is a conserved UFL1-interacting protein in mammals. However, there is no study on UFBP1 in silkworm yet. In this study, we identified a UFBP1 ortholog in B. mori genome. Spatio-Temporal expression profiles showed that BmUFBP1 expression was highly in midgut, fatbody, and at the moth stage. BmUFBP1 knockdown inhibited ER-stress induced ER chaperone expression and BmNPV proliferation, while BmUFBP1 overexpression increased BmNPV proliferation. Furthermore, RNA sequencing and experimental verification identified the HSP70 family member BmBIP as the BmUFBP1 downstream target in regulating BmNPV proliferation. Overall, these results suggest that BmUFBP1 facilitates BmNPV proliferation via ATF6-BIP signaling.

2024-07-31 | GSE272804 | GEO

Genome wide microarray based expression profiles associated with BmNPV resistantance and susceptibility in Indian silkworm races of Bombyx mori

Project description:The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in the midgut tissues of BmNPV infected resistant (Sarupat) and susceptible (CSR-2) Indian silkworm races. In the resistant race, 735 genes were upregulated and 589 genes were down regulated at 12 hours of BmNPV post infection. Similarly in case of susceptible race, 2183 genes were up regulated and 2115 genes were down regulated. Among the differentially expressed genes, nine upregulated and eight down regulated genes were validated using real time qPCR analysis. In Sarupat, the significantly upregulated genes are vacuolar protein sorting associated gene, X fin like protein and Carboxy peptidase E like protein in BmNPV infected midguts, whereas the prominent down regulated genes are glutamate receptor ionotropic kainite 2-like, BTB/POZ domain and transferrin. In the case of CSR-2, the considerably upregulated genes are Peptidoglycan recognition protein S6 precursor and rapamycin while the conspicuous down regulated genes are facilitated trehalose transporter and zinc transporter ZIP1-like gene. Our results provided a vital insights of Bombyx mori in reference with its molecular mechanism in immune response against the BmNPV invasion. Organism : Bombyx mori , Agilent Custom Silkworm Gene Expression 4x44k Array (AMADID: 066047) designed by Genotypic Technology Private Limited.

2016-07-01 | E-GEOD-71240 | biostudies-arrayexpress

Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (Bombyx mori) Cells

Project description:Bombyx mori is one of the key lepidopteran model species, and is economically important for silk production and proteinaceous drug expression. Baculovirus and insect host are important natural biological models for studying host-pathogen interactions. The impact of Bombyx mori nucleopolyhedrovirus (BmNPV) infection on the proteome and acetylome of Bombyx mori ovarian (BmN) cells were explored to facilitate a better understanding of infection-driven interactions between BmNPV and host in vitro. The proteome and acetylome were profiled through 6-plex Tandem mass tag (TMT) labelling-based quantitative proteomics. Totally, 4,194 host proteins were quantified, of which 33 were up-regulated and 47 were down-regulated in BmN cells at 36 h post-infection. Based on the proteome, quantifiable differential Kac proteins were identified and functionally annotated to gene expression regulation, energy metabolism, substance synthesis and metabolism after BmNPV infection. Altogether, 644 Kac sites in 431 host proteins and 39 Kac sites in 22 viral proteins were identified and quantified in infected BmN cells. Our study demonstrated that BmNPV infection globally impacts the proteome and acetylome of BmN cells. The viral proteins are also acetylated by the host acetyltransferase. Protein acetylation is essential for cellular self-regulation and response to virus infection. This study provides new insights for understanding the host-virus interaction mechanisms, and the role of acetylation in BmN cellular response to viral infection.

2017-11-27 | PXD008054 | Pride

Genome wide microarray based expression profiles associated with BmNPV resistantance and susceptibility in Indian silkworm races of Bombyx mori

2016-07-01 | GSE71240 | GEO

Changes in gene expressions associated with egg diapause in multivoltine silkworm Bombyx mori

Project description:Changes in gene expressions associated with egg diapause in multivoltine silkworm Bombyx mori

2012-09-30 | GSE35622 | GEO

Changes in gene expressions associated with egg diapause in multivoltine silkworm Bombyx mori

Project description:Changes in gene expressions associated with egg diapause in multivoltine silkworm Bombyx mori Agilent one-color experiment, Agilent-Custom : Bombyx mori 4x44k designed by Genotypic Technology Pvt. Ltd. (AMADID: 034793) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0444)

2012-09-30 | E-GEOD-35622 | biostudies-arrayexpress

Bombyx mori

Project description:Bombyx mori

| PRJNA715974 | ENA

Bombyx mori

Project description:Bombyx mori

| PRJNA783725 | ENA

Dosage compensation in Bombyx mori is achieved by partial repression of both Z chromosomes in males

Project description:We report the ATAC-seq data that reveal chromatin accessibility during Bombyx mori development

2022-02-05 | GSE191164 | GEO

Bombyx mori

Project description:Bombyx mori Metagenome