Project description:To measure natural variation in ER stress transcriptional response in a subset of lines from the Drosophila Genetic Reference Panel
2013-04-30 | GSE46425 | GEO
Project description:Aging in the Drosophila Genetic Reference Panel (fruit fly)
Project description:In order to gain insights into gut immunocompetence and its variation in a population, we performed RNA-sequencing of whole guts of 38 Drosophila Genetic Reference Panel strains.
Project description:Profiling of histone modifications with the iChIP protocol (PMID25103404) using antibodies against two markers of regulatory activity: H3K27ac (abcam, ab4729) and H3K4me3 (Millipore, 07-473). All paternal fly lines were taken from the Drosophila Genetic Reference Panel crossed to a common mother (PMID31308546). Data were collected at three time points (2-4h, 6-8h, 10-12h at 25C) with two biological replicates per collection.
Project description:Sequencing of mRNAs of 22 wild type strains from the Drosophila Genetic reference Panel (DGRP) at embryonic time point 10-12 hrs after egg laying was performed. ~100 embryos were pooled for each biological sample prior to library preparation.
