Project description:human somatic cell MRC-5 had different gene expression level compared with that of the adult cells including CCD-1079sk and IMR90. This difference gave the human somatic cell MRC-5 unique characteristics which could then be compared with the human ES cells by genes comparison to show the gene expression difference between human somatic cells and human ES cells.
Project description:Human ES cells had different gene expression level compared with that of the adult cells including CCD-1079sk and IMR90. This difference gave the human ES cells unique characteristics which could then be compared with other species' ES cells or iPS cells like mouse ES cells and Rat iPS cells by the homologue genes comparison to show which species' ES cells or iPS cells were more competitive with the other.
Project description:Remaining adult bees in colonies suffering from CCD at apiaries in Florida, California and Pennsylvania were collected during the winter of 2006-2007. The health of CCD colonies was scored at the time of collection as either ï¾severeï¾ or ï¾mildï¾ depending on the apparent strength of the colony. ï¾Historicalï¾ bees, collected prior to the appearance of CCD and hence ostensibly healthy, were collected in 2004 and 2005 from colonies set up on new equipment and not receiving any miticide treatments from apiaries of The Pennsylvania State University near State College, PA. A combination looped, common-reference microarray design was used to compare historical and CCD samples with each other; a blend of RNA isolated from healthy colonies (collected near Urbana, IL in July 2007) served as reference. The microarray experiment compared the guts of bees from mildly and severely afflicted colonies collected in apiaries experiencing CCD on the East Coast (Florida and Pennsylvania) and West Coast (California) with a common reference.
Project description:Rat somatic lung cell had different gene expression level compared with that of the adult cells including rat bone marrow cells(BMC) and rat primary ear fibroblasts(PEF). This difference gave the rat somatic lung cell unique characteristics which could then be compared with the rat iPS cells by genes comparison to show the gene expression difference between rat somatic lung cells and rat iPS cells.
