Project description:To identify novel miRNA and NAT-siRNAs that are associated with salt and cold stresses in Arabidopsis, we generated small RNA sequences from Arabidopsis plants under salt and cold stress treatments. Sequencing of small RNAs in Arabidopsis under salt, and cold stress conditions.
Project description:To identify novel miRNA and NAT-siRNAs that are associated with salt and cold stresses in Arabidopsis, we generated small RNA sequences from Arabidopsis plants under salt and cold stress treatments.
Project description:To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in Arabidopsis, we generated small RNA sequences from adult Arabidopsis plants under control and under dought, salt, and cold stress treatments. Over 23 million reads were generated. Sequencing of small RNAs in Arabidopsis under control, drought, salt, and cold stress conditions.
Project description:In Arabidopsis, CBFs transcription factors (CBF1, CBF2 and CBF3) play fundamental roles in plant cold tolerance, especially in cold-acclimation. By employing CRISPR/Cas9, we knocked out CBF1 and CBF2 simultaneously in cbf3 background and generated cbfs triple mutant. This mutant facilitated the discovery of CBF-regulated genes. In this study, Arabidopsis 14-d-old Col-0 and cbfs mutant were treated with cold stress (4 degree centigrade) for 0, 3 and 24 hours. The seedlings were harvested for total RNA extraction and sequencing.
