Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1139387 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR30001058.fastq.gz | Fastqsanger.gz | |||
| SRR30001059.fastq.gz | Fastqsanger.gz | |||
| SRR30001060.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 3 of 3 |
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Project description:Leaf colour variation is observed in several plants. We obtained two types of branches with yellow (H1) and variegated (H2) leaves from Camellia sinensis. To reveal the mechanisms that underlie the leaf colour variations, proteomic analysis using label-free MS-based approach was performed using leaves from variants and normal branches (CKs).
2018-10-26 | PXD004750 | Pride
Project description:In order to investigate the physiological and biochemical characteristics and molecular mechanisms during the leaf colour change of Acer rubrum L, this study used Acer rubrum L. 'Autumn Blaze' cuttings as material and analysed the transcriptome and miRNAs of Acer rubrum L leaves under different light and temperature treatments. The transcriptome and miRNAs of Acer rubrum L leaves were analysed under different light and temperature treatments, and miRNA-mRNA association analysis was performed for the differentially expressed mRNAs and miRNAs.
2023-03-02 | GSE226127 | GEO
Project description:<p><strong>BACKGROUND:</strong> <em>Loropetalum chinense </em>var.<em> rubrum</em> (<em>L. chinense</em> var. <em>rubrum</em>) is a precious, native, coloured-leaf ornamental plant in the Hunan Province. We found a <em>L. chinense</em> var. <em>rubrum</em> tree with 3 different leaf colours: GL (green leaf), ML (mosaic leaf) and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. The aim of this study was to identify the metabolites and genes involved in determining the colour composition of <em>L. chinense</em> var. <em>rubrum</em> leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.</p><p><strong>RESULTS:</strong> We observed that the mesophyll cells in PL were purple, those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed that the differences in content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonin and petunidin 3,5-diglucoside in ML, GL and PL were significant. Considering that the trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of <em>L. chinense</em> var. <em>rubrum</em> leaves. Finally, using transcriptomics, we identified 9 differentially expressed structural genes (one <em>ANR</em> (<em>ANR1217</em>); four <em>CYP75A</em> (<em>CYP75A1815</em>, <em>CYP75A2846</em>, <em>CYP75A2909</em> and <em>CYP75A1716</em>); four UFGTs (<em>UFGT1876</em>, <em>UFGT1649</em>, <em>UFGT1839</em> and <em>UFGT3273</em>) and nine transcription factors (two <em>MYBs</em> (<em>MYB1057</em> and <em>MYB1211</em>), one <em>MADS-box</em> (<em>MADS1235</em>), two <em>AP2-likes</em> (<em>AP2-like1779</em> and <em>AP2-like2234</em>), one <em>bZIP</em> (<em>bZIP3720</em>), two <em>WD40s</em> (<em>WD2173</em> and <em>WD1867</em>) and one <em>bHLH</em> (<em>bHLH1631</em>) that might be related to flavonoid biosynthesis that impacted the appearance of colour in <em>L. chinense</em> var. <em>rubrum</em> leaves.</p><p><strong>CONCLUSIONS:</strong> This study revealed potential molecular mechanisms associated with leaf coloration in <em>L. chinense</em> var. <em>rubrum</em> by analysing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provides a reference for research on leaf colour variation in other ornamental plants.</p>
2023-01-30 | MTBLS5662 | MetaboLights
Project description:Time series response of potato cv. Désirée, which is tolerant to PVY infection, was analysed in both inoculated as well as upper non-inoculated leaves. Additionally, transgenic plants deficient in accumulation of salicylic acid (NahG- Désirée) were studied in the same setting. 2 genotypes, mock and virus inoculated plants, inoculated (1-7dpi) and non-inoculated (1-11 dpi) leaves; one-colour design
2015-08-06 | E-GEOD-58593 | biostudies-arrayexpress
Project description:Tillandsia landbeckii Genome sequencing
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Project description:Background: Heliconius butterflies are an excellent model system for studies of adaptive convergent and divergent phenotypic traits. Wing colour patterns are used as signals to both predators and potential mates and are inherited in a Mendelian manner. The underlying genetic mechanisms of pattern formation have been studied for many years and shed light on broad issues, such as the repeatability of evolution. In Heliconius melpomene, the yellow hindwing bar is controlled by the HmYb locus and several genes in this region show expression pattern differences across races. MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that have key roles in many biological processes, including development. It seems likely that miRNAs could act as downstream regulators of genes involved in wing development, patterning and pigmentation. For this reason we characterised miRNAs in developing butterfly wings and examined differences in their expression between colour pattern races. Results: We sequenced small RNA libraries from two colour pattern races and detected 142 Heliconius miRNAs with homology to others found in miRBase. Several highly abundant miRNAs appeared to be differentially expressed between colour pattern races and this was tested further in different developing pupal wing stages using Northern blots. These revealed that differences in expression were due to developmental stage rather than colour pattern. Assembly of sequenced reads to the HmYb region identified miR-193 and miR-2788; located 2380bp apart in an intergenic region. A search for miRNAs in all available H. melpomene BAC sequences (~2.5Mb) did not reveal any other miRNA genes and no novel miRNAs were predicted. There were several regions where other small RNA sequences assembled to the HmYb region and appeared to be differentially expressed.These might represent other regulatory RNAs. Conclusions: Here we describe the first butterfly miRNAs and characterise their expression in developing wings. Some show differences in expression across developing pupal stages. Two miRNAs were located in the HmYb region. Future work will examine the expression of these miRNAs in different colour pattern races and identify miRNA targets among wing patterning genes.
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