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A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique signatures in blood T cell-monocyte complexes during infection

ABSTRACT: A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique signatures in blood T cell-monocyte complexes during infection

PROVIDER: PRJNA1139790 | ENA |

REPOSITORIES: ENA

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A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (Dengue scRNA-Seq)

Project description:We designed a novel method to study the transcriptome of single cells forming complexes in a high-throughput fashion, without the need for bioinformatic deconvolution. We applied this method to the study of T cells and monocytes forming complexes in blood isolated from individuals with dengue fever, with blood collected at the acute and convalescent phase of infection. We found that the transcriptomic signatures of T cells and monocytes forming complexes significantly overlapped with those identified in T cell-monocyte complexes in active tuberculosis, with upregulation of genes associated with T cell activation, cytotoxicity, cell adhesion and MHC-II. Thus, using our novel method, we identified that T cells and monocyte forming complexes in blood hold unique immune signatures distinct from singlets, and that they may be a previously overlooked valuable biomarker to monitor immune synaptic interactions during infection.

2024-11-06 | GSE273017 | GEO

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (Dengue scTCR-Seq)

2024-11-06 | GSE273018 | GEO

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (ATB scTCR-Seq)

Project description:We designed a novel method to study the transcriptome of single cells forming complexes in a high-throughput fashion, without the need for bioinformatic deconvolution. We applied this method to the study of T cells and monocytes forming complexes in blood isolated from individuals with active tuberculosis, with blood collected at diagnosis and after treatment. We found that the transcriptomic signature of T cells and monocytes forming complexes was associated with TCR/MHC-II immune synaptic features, enrichment for cytotoxic clonally expanded T cells and intermediate monocytes, and - especially at diagnosis - transcriptomic signatures indicating increased immune and metabolic activity. We also found a striking enrichment of “dual-expressing” cells (i.e., co-expressing T cells and monocyte marker genes) in complexes, suggesting RNA exchange occur during T cell-monocyte interactions. Thus, using our novel method, we identified that T cells and monocyte forming complexes in blood hold unique immune signatures distinct from singlets, and that they may be a previously overlooked valuable biomarker to monitor immune synaptic interactions during infection.

2024-11-06 | GSE273016 | GEO

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (ATB scRNA-Seq)

Project description:We designed a novel method to study the transcriptome of single cells forming complexes in a high-throughput fashion, without the need for bioinformatic deconvolution. We applied this method to the study of T cells and monocytes forming complexes in blood isolated from individuals with active tuberculosis, with blood collected at diagnosis and after treatment. We found that the transcriptomic signature of T cells and monocytes forming complexes was associated with TCR/MHC-II immune synaptic features, enrichment for cytotoxic clonally expanded T cells and intermediate monocytes, and - especially at diagnosis - transcriptomic signatures indicating increased immune and metabolic activity. We also found a striking enrichment of “dual-expressing” cells (i.e., co-expressing T cells and monocyte marker genes) in complexes, suggesting RNA exchange occur during T cell-monocyte interactions. Thus, using our novel method, we identified that T cells and monocyte forming complexes in blood hold unique immune signatures distinct from singlets, and that they may be a previously overlooked valuable biomarker to monitor immune synaptic interactions during infection.

2024-11-06 | GSE273015 | GEO

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (Dengue scRNA-Seq)

Project description:A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (Dengue scRNA-Seq)

| PRJNA1139796 | ENA

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (Dengue scTCR-Seq)

| PRJNA1139795 | ENA

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (ATB scTCR-Seq)

| PRJNA1139797 | ENA

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique immune signatures in blood T cell-monocyte complexes during infection (ATB scRNA-Seq)

| PRJNA1139794 | ENA

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique signatures in blood T cell-monocyte complexes during infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-11-06 | GSE273019 | GEO

Distinct blood CD3+ CD14+ T Cell-Monocyte complexes harbor HIV and are dynamic, glucose-dependent and increased in individuals with glucose intolerance

Project description:Persistent systemic inflammation in persons with HIV (PWH) is accompanied by an increased risk of metabolic and cardiovascular diseases. Yet, changes in the innate and the adaptive immune in PWH who develop cardiometabolic disease remain insufficiently defined. Using mass cytometry we showed that PWH with prediabetes/diabetes had a significantly higher proportion of circulating CD14+ monocytes complexed with T cells. The CD3+ T cells and CD14+ monocytes consisted of dynamic heterogeneous complexes. Furthermore, we detected more HIV DNA in T cell-monocyte complexes compared to CD14+ monocytes or CD4+ T cells alone. Our results demonstrate that CD3+ CD14+ T cell-monocyte pairs are functional and physically interacting immune cell complexes that are higher among PWH with diabetes, suggesting that they may contribute to metabolic disease pathogenesis, and provide a strong incentive for future studies to investigate T cell-monocyte immune complexes as mechanistic in HIV cure reservoir and other diseases of aging.

2024-12-31 | GSE230276 | GEO

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