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Expression changes in mouse proximal tubules during primary culture

ABSTRACT: Expression changes in mouse proximal tubules during primary culture

PROVIDER: PRJNA1139850 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29978534_1.fastq.gz	Fastqsanger.gz
	SRR29978534_2.fastq.gz	Fastqsanger.gz
	SRR29978535_1.fastq.gz	Fastqsanger.gz
	SRR29978535_2.fastq.gz	Fastqsanger.gz
	SRR29978536_1.fastq.gz	Fastqsanger.gz

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Expression changes in mouse proximal tubules during primary culture

Project description:Primary cultures of proximal tubule cells are widely used to model the behavior of kidney epithelial cells in vitro. De-differentiation of primary cells upon culture has been observed and appreciated for decades, yet the mechanisms driving this phenomenon remain poorly understood. The goal of this experiment was to quantitatively measure the changes in expression of purified proximal tubules as they enter primary culture.

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Transcription profiling of mouse Clcn5 knockout in proximal tubules of the kidney

Project description:Dent disease has multiple defects attributed to proximal tubule malfunction including low molecular weight proteinuria, aminoaciduria, phosphaturia and glycosuria. In order to understand the changes in kidney function of the Clc5 transporter gene knockout mouse model of Dent disease, we examined gene expression profiles from proximal tubules of mouse kidneys. Overall 720 genes are expressed differentially in the proximal tubules of the Dent Clcn5 knockout mouse model compared to those of control wild type mice. The fingerprint of these gene changes may help us to understand the phenotype of Dent disease. Experiment Overall Design: Renal proximal tubules were dissected from wild type and Clcn5 knockout mice. Mice were anesthetized with halothane, the abdominal aorta of each animal was accessed and the left kidney was perfused with an ice-cold salt. Proximal tubule dissection was performed in an ice-cold salt solution. After dissection of approximately 80-100 segments of 2 mm in length per kidney, the RNA for 3-4 mice was combined to have enough RNA per chip. Experiment Overall Design: 3 microarrays each of wild type and knockout mouse proximal tubule were processed

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Expression data from 20-week-old mice proximal tubules

Project description:dB/dB mice develop different kidney pathologies resulting from high body weight. We seek to better understand the mechanisms of this kidney damage in proximal tubules. We isolated RNA from proximal tubules of different groups of mice, and we seek to understand how the anti-oxidant enzyme catalase may regulate kidney damage in this model.

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Project description:Pax2 and Pax8 are homologous transcription factors required for kidney development and medullary urine concentration. However, their function in proximal tubule homeostasis and response to acute kidney injury is unknown. Mice with proximal tubules consisting of a mosaic of wild-type and Pax2/8 mutant proximal tubules cells were generated. Gene expression of mutant and wild-type proximal tubule cells was compared under homeostatic conditions using single-nucleus RNA sequencing.

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