Project description:To investigate transcriptomic profiles when auxin-dependent transcription factor ARFC2 from Physcomitrium patens is expressed, we established an inducible overexpressor line for PpARFC2. We then perform bulk RNA-seq on protonemata tissues that have been mock-treated or induced by beta-estradiol for 16 h.
2022-09-27 | GSE213920 | GEO
Project description:Bulk RNA-seq of Physcomitrium patens treated by cytokinin
Project description:Physcomitrium (Physcomitrella) patens Gransden wild type strain and four mutants Δdek1 (Perroud et al., 2014); Δloop (Demko et al., 2014); Δlg3 (Johansen et al., 2016) and oex1 (this work) were sequenced for time series analysis.
Project description:We studied peptide generation process in moss Physcomitrella patens. For this purpose, analysis of peptidome and transcriptome of two different life forms (gametophores and protonema) and also of protoplasts were made.
Project description:Protonema of Physcomitrella patens Pp_A_Doko#069 (DOKO69; Koprivova et al., 2004) grown in liquid cultures with Knop medium (Reski and Abel, 1985) were harvested 6 days after last subculture and setting density to 120 mg/L dry weight.