Project description:Next generation sequencing was performed to identify genes changed in Colletotrichum camelllae upon infection tea plants. The goal of the work is to find interesting genes involved in fungal virulence. The object is to reveal the molecular mechanism of funal virulence.
2023-01-25 | GSE205689 | GEO
Project description:Fungal community diversity in Wuyishan oolong tea growing area
Project description:Pu-erh tea has attracted increasing attention worldwide because of its special flavor and health effects, but its impact on composition and function of the gut microbiota remains unclear. The aim of this study was to investigate effects of aqueous extracts of fermented (ripe) and non-fermented (raw) Pu-erh teas on the composition and function of intestinal microbiota of rats with diet-induced obesity. We conducted a comparative metagenomic and metaproteomic investigation of the microbial communities in cecal samples taken from obese rats administrated with or without extracts of raw and ripe Pu-erh tea. By analyzing the composition and diversity of 16S rRNA amplicons and expression profiles of 814 distinct proteins, we found that, despite differences in the chemical compositions of the raw and ripe Pu-erh tea, administration of either at two different doses (0.15 and 0.40 g/Kg body weight), significantly (P<0.05) increased community diversity, and changed the composition of the cecal microbiota by increasing the relative abundances of Firmicutes and decreasing those of Bacteroidetes. Community metabolic processes including sucrose metabolism, glycolysis, syntheses of proteins, rRNA and antibiotics were significantly (P<0.05), or had a tendency (0.10<P<0.05) to be, promoted by enriching relevant enzymes. Furthermore, evidences from population, molecular and metabolic levels have shown that polyphenols of raw Pu-erh tea and their metabolites can promote potentially the growth of Akkermansia municiphila by stimulating the type II and III secretion system protein, elongation factor Tu, and glyceraldehyde-3-phosphate dehydrogenase. This study has provided new evidences for the prebiotic effects of Pu-erh tea.
2022-03-03 | PXD010972 | Pride
Project description:tea phyllosphere fungal community
| PRJNA657458 | ENA
Project description:The fungal diversity and community structure in Chinese Fuzhuan brick tea
| PRJNA560449 | ENA
Project description:Fungal communities of dark tea
Project description:<p>Entomopathogenic fungi have the ability to both directly kill insect pests and act as plant endophytic fungi to impact plant growth and development. Despite this, the widespread endophytic use of these fungi in tea plants (Camellia sinensis) is still limited. This study examined how Beauveria bassiana colonizes tea plant tissues and its impact on tea plant growth and development. Through amplicon sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS), the study investigated the response patterns of dominant endophytic microbial populations in tea plants during fungal colonization and the alterations in secondary metabolites in tea plants due to the colonization process. B. bassiana effectively colonized tea seedlings through root irrigation and foliar spraying methods, showing a preference for stems, and the colonization persisted for more than 90 days. Colonization resulted in a reduction in the diversity and structural stability of the endophytic microbial community in tea plants; however, it also enhanced the importance of ecologically mutualistic relationships or cooperative interactions in community assembly. Additionally, colonization had a more pronounced effect on endophytic fungi compared to endophytic bacteria. It led to an increase in the relative abundance of arbuscular mycorrhizal fungi in root tissues and a decrease in the relative abundance of total plant pathogens in tea plant tissues. Terpenoids were the most significantly associated differential metabolites following B. bassiana colonization, followed by steroids and their derivatives, and flavonoids. Colonization by B. bassiana resulted in elevated levels of the majority of differential metabolites in tea plant stems at the onset of colonization. The colonization of B. bassiana in tea plants displayed a strong positive correlation with 26 distinct metabolites, such as proanthocyanidin B2 and L-malic acid. This colonization altered the endophytic microbial community, impacting metabolic pathways associated with plant hormone synthesis, volatile compound production, as well as the growth, development, and defense of tea plants.</p>
Project description:Keemun black tea is a fully fermented tea. These LC-MS data help us know the chemical diversity during processing of Keemun black tea.