Project description:This SuperSeries is composed of the following subset Series:; GSE12402: Expression data from Arabidopsis seed compartments at the pre-globular stage. GSE12403: Expression data from Arabidopsis seed compartments at the linear-cotyledon stage. Experiment Overall Design: Refer to individual Series
Project description:We collected bending cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear cotyledon stage embryos. For the purposes of this study we captured 5 compartments: embyro proper, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire linear cotyledon stage seed. Bending cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
Project description:We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 5 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat and general seed coat. Experiment Overall Design: linear-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 10 samples (embryo proper, micropylar endosperm, cellularized endosperm, general seed coat and chalazal seed coat, 2 biological replicates each ).
Project description:We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 7 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat, general seed coat, whole seeds and micropylar endosperm.
Project description:We collected bending-cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing bending cotyledon stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, mycropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire bending-cotyledon stage seed.
