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ABSTRACT: Northwestern Medicine Candida auris NGS

PROVIDER: PRJNA1144841 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30146020_1.fastq.gz	Fastqsanger.gz
	SRR30146020_2.fastq.gz	Fastqsanger.gz
	SRR30146021_1.fastq.gz	Fastqsanger.gz
	SRR30146021_2.fastq.gz	Fastqsanger.gz
	SRR30146022_1.fastq.gz	Fastqsanger.gz

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Potent synergistic interactions between lopinavir and azole antifungal drugs against emerging multidrug-resistant Candida auris

Project description:The limited number of antifungals and the emergence of multidrug-resistant Candida auris pose a significant challenge to human medicine. Here, we utilized combinatorial drug therapy as an approach to augment the activity of current azole antifungals against C. auris. We evaluated the fluconazole chemosensitization activity of 1547 FDA-approved drugs and clinical molecules against an azole-resistant strain of C. auris. This led to the discovery that lopinavir, an antiviral drug, is a potent agent capable of sensitizing C. auris to the effect of azole antifungals. At a therapeutically achievable concentration (4-8 µg/ml), lopinavir exhibited potent synergistic interactions with azole drugs, particularly with itraconazole, against C. auris (ΣFICI ranged from 0.05-0.50). The lopinavir/itraconazole combination enhanced the survival rate of C. auris-infected Caenorhabditis elegans by 90% and reduced the fungal burden in infected nematodes by 88.5% (p < 0.05). Moreover, lopinavir enhanced the antifungal activity of itraconazole against other medically important Candida species including C. albicans, C. tropicalis, C. glabrata, C. tropicalis, and C. parapsilosis. Comparative transcriptomic profiling revealed that lopinavir interferes with glucose permeation and ATP synthesis. This compromises the function of the efflux pumps presents in C. auris enhancing sensitivity to azole antifungals, as demonstrated by Nile red efflux assays. This study presents lopinavir as a novel, potent and broad-spectrum azole chemosensitizing agent that warrants further investigation against recalcitrant Candida infections.

2020-10-06 | GSE148341 | GEO

High-throughput RNA Sequencing of BMDM of Mus musculus stimulated by C. auris or C. albicans

Project description:Candida auris has been globally recognized as a multidrug-resistant human fungal pathogen that contributes for the worldwide occurrence of nosocomial outbreaks. It has been reported that C. auris was able to avoid neutrophil attack, suggestive of an impaired innate immune response. Whether C. auris evades the innate immune recognition of BMDM (bone marrow derived macrophage) remains poorly understood, and as for well-known Candida species -C. albicans, it can trigger immune response. To determine whether occurs difference between immune response stimulated by C. auris or C. albicans, we performed mRNA-seq of BMDM stimulated by C. auris or C. albicans.

2022-05-21 | GSE203508 | GEO

Transcriptomics and phenotyping define signatures associated with echinocandin resistance in Candida auris

Project description:Transcriptomic analysis of different Candida auris clinical isolates

2022-03-16 | GSE198410 | GEO

Haploid and Deploid for Candida auris

Project description:The expression difference for haploid and deploid of Candida auris

2020-09-13 | GSE149284 | GEO

Transcriptional Signatures Predict Phenotypic Variations of Candida auris

Project description:Transcriptomic analysis of different Candida auris clinical isolates with distinct antifungal susceptibility profiles.

2021-01-30 | GSE165762 | GEO

Whole genome sequencing of tebuconazole adaptors in Candida auris

Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.

2023-05-10 | E-MTAB-12802 | biostudies-arrayexpress

FY279-TUN-8ug/ml adaptors

Project description:Approximately 1 million cells of Candida auris isolate FY279 were spread on YPD plate supplemented with 8ug/ml tunicamycin. Randomly 21 adaptors were chosen. These adaptors were sequenced.

2023-08-20 | E-MTAB-12496 | biostudies-arrayexpress

Candida auris derived tunicamycin adaptors using high amount of tunicamcyin

Project description:Candida auris reference strain B11221 was spread on YPD plate supplemented with 8 μg/ml tunicamycin. Randomly 25 adaptors (T2080-T2104) were chosen. These adaptors and the parent were sequenced.

2023-10-05 | E-MTAB-12665 | biostudies-arrayexpress

Development and Validation of an in-house library with Colombian Candida auris strains in MALDI TOF MS to improve yeast identification

Project description:Creation of a new library entries for Candida auris using MALDI Biotyper. Candida auris has a high genetic variability in the world, the identification of Colombian isolates is difficult using the main Bruker library. A new in-house library was created using Colombian isolated and was validated using 300 isolated strains

2020-06-02 | PXD016387 | Pride

Identification of the most immunoreactive antigens of Candida auris to sera from infected mice

Project description:Candida auris is a recently found pathogenic yeast that causes systemic infections, showing a high mortality rate. The delay on making a correct diagnosis of C. auris is a current problem in the healthcare system setting. As immunoproteomics studies are important to identify immunoreactive proteins for new diagnostic strategies, in this study immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris, and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The aim was to identify the most immunoreactive antigens of this yeast. Thirteen spots were recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry. They corresponded to enolase, phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase and phosphoglycerate mutase. These four proteins seem to be also recognized by sera obtained from human patients with disseminated C. auris infection, but not by sera obtained from mice infected with other fungi such as C. albicans or Aspergillus fumigatus. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris.

2024-06-23 | PXD049077 | Pride

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