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Drosophila melanogaster

ABSTRACT: The effects of PTIP on Polycomb mediated repression in Drosophila Kc167 cells.

OTHER RELATED OMICS DATASETS IN: E-GEOD-15092

PROVIDER: PRJNA114797 | ENA |

REPOSITORIES: ENA

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The effects of PTIP on Polycomb mediated repression in Drosophila Kc167 cells.

Project description:Drosophila Kc167 cells were used to test the function of PTIP in regulating gene expression before and after loss of polycomb function. Four condtions were tested using dsRNA knockdowns (RNAi) Experiment Overall Design: Kc167 cells alone, Kc cells with loss of PTIP, cells with loss of polycomb and polyhomeotic, and cells with loss of PTIP, polycomb, and polyhomeotic. Performed in triplicate.

2009-03-15 | E-GEOD-15092 | biostudies-arrayexpress

E-GEOD-15092

Project description:The effects of PTIP on Polycomb mediated repression in Drosophila Kc167 cells.

2014-10-08 | E-GEOD-15092 | ExpressionAtlas

The effects of PTIP on Polycomb mediated repression in Drosophila Kc167 cells.

2009-03-04 | GSE15092 | GEO

Drosophila melanogaster

Project description:Distribution of Drosophila insulator proteins DREF in Drosophila Kc167 cells

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Knockdown of Polyhomeotic in Drosophila Kc cells

Project description:In this experiment, we used RNAi targeting both copies of the gene polyhomeotic (ph-p and ph-d) to knockdown expression of this factor in Drosophila Kc167 cells. We observed substantial changes to the structure of genomic loci bound by Polycomb-group proteins as assayed by single-molecule based super-resolution imaging with STORM. We also observed a substantial de-repression of many genes which reside in Polycomb-bound domains, as assayed with RNA-seq. mRNA profiles from Drosophila Kc167 cells with and without knockdown the genes ph-p and ph-d were examined in duplicate.

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Knockdown of Polyhomeotic in Drosophila Kc cells

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Project description:Histone Phosphoacetylation ChIP-seq of Kc167 cells from Drosophila

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Project description:modENCODE RNA-Seq of Drosophila Kc167

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Project description:TAP-ORC2 and control ChIP experiments in Drosophila Kc167 cells

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