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RNA sequencing of MC38 tumor cells from control-diet- and high-fat-diet-fed C57BL/6J mice

ABSTRACT: RNA sequencing of MC38 tumor cells from control-diet- and high-fat-diet-fed C57BL/6J mice

PROVIDER: PRJNA1148134 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30248827.fastq.gz	Fastqsanger.gz
	SRR30248828.fastq.gz	Fastqsanger.gz
	SRR30248829.fastq.gz	Fastqsanger.gz
	SRR30248830.fastq.gz	Fastqsanger.gz
	SRR30248831.fastq.gz	Fastqsanger.gz

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Expression data from livers of C57BL/6J mice fed a high-fat diet and coffee

Project description:The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. Briefly, 7-week-old male C57BL/6J mice purchased from Charles River Laboratories Japan (Yokohama) were divided into the following five groups. The normal diet group (ND group) was fed D12450B (10 kcal% fat, Research Diets, New Brunswick, NJ, USA). The high-fat diet group (HF group) was fed D12492 (60 kcal% fat, Research Diets, New Brunswick, NJ, USA). The caffeinated coffee group (HFCC group) was fed a high-fat diet containing 2% caffeinated freeze-dried coffee. The decaffeinated coffee group (HFDC group) was fed a high-fat diet containing 2% decaffeinated freeze-dried coffee. The green unroasted coffee group (HFGC group) was fed a high-fat diet containing 2% unroasted caffeinated freeze-dried coffee. The mice had ad libitum access to their diets and drinking water. After 9 weeks, mice were sacrificed and the livers were subjected to the Affymrtix DNA microarray experiment.

2013-12-10 | E-GEOD-53131 | biostudies-arrayexpress

Expression data from livers of C57BL/6J mice fed a high-fat diet and coffee

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Project description:Obesity is a major cancer risk factor, but the underlying molecular mechanisms are not always known. In this study, we look at proteome remodeling in cancer cells with obesity, comparing tumor cells sorted from mice fed high-fat versus control diet. We conducted 10-plex TMT-proteomics on GFP+ MC38 colorectal adenocarcinoma tumor cells, sorted from subcutaneously implanted tumors 12 days after implantation. This study reveals molecular pathways that change in cancer cells with obesity that promote tumor growth.

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Transcriptional analysis of CD45+ leukocytes isolated from syngeneic MC38 tumors from mice fed high-fat or control diet

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