Project description:This SuperSeries is composed of the following subset Series: GSE15047: Nasopharyngeal carcinoma cell lines (expression analysis) GSE15051: Nasopharyngeal carcinoma cell lines (copy number analysis) This study was designed to screen genes overexpression due to copy number gain.
Project description:To analyze the global copy number aberrations of two nasopharyngeal carcinoma cell lines, TW01 and HONE1. Global copy number aberrations were analyzed by using high-resolution oligoarray CGH on two NPC cell lines, TW01 and HONE1. The overviews of array CGH profiles reveal high similarity between both NPC cell lines, but the degree of copy-number alterations were more severe in TW01 than in HONE1. There were 1204 and 1513 genes with copy-number gain (CNVs with aberration score > 0.5 and number of contiguous probes ≥ 3) in TW01 and HONE1, respectively. Among them, 850 were commonly amplified in both cell lines (Gain-TH). There were 3525 genes and 926 genes with copy-number loss (CNVs with aberration score < 0.5 and number of contiguous probe ≥ 3) in TW01 and HONE1, respectively. Among them, 582 were commonly deleted in both cell lines (Loss-TH). The most prominent CNVs observed including gain on 3q26.2-q26.31, loss on 3p21.2-q12.1, 9p24.3-p21.3, and nearly the whole Y chromosome.
Project description:The comprehensive DNA methylation analysis with nasopharyngeal carcinoma cases with normal nasopharyngeal epithelial tissues, and nasopharyngeal epithelial cell lines. Infinium HumanMethylation850 BeadChip was used to obtain DNA methylation profiles across 850,000 CpG sites. Samples included 2 nasopharyngeal carcinoma cases, 3 normal nasopharyngeal epithelial tissues, and NP69T and NP69T-LMP1 cell lines.
Project description:Five NPC cell lines (HONE1, CNE1, CNE2, 5-8F, and 6-10B) and an immortalized nasopharyngeal epithelial cell line NP69 were evaluated with 250K SNP arrays (Affymetrix) to detect the genome-wide DNA copy number alterations.
Project description:84 NSCLC cell lines were collected from various sources (Supplemental Table 1) and formed the basis for all subsequent experiments. Cell lines were derived from tumors representing all major subtypes of NSCLC tumors, including adenocarcinoma, squamous-cell carcinoma and large-cell carcinoma. The genomic landscape of these cell lines was characterized by analyzing gene copy number alterations using high-resolution single-nucleotide polymorphism (SNP) arrays (250K Sty1). We used the statistical algorithm Genomic Identification of Significant Targets in Cancer (GISTIC) to distinguish biologically relevant lesions from background noise. The application of GISTIC revealed 16 regions of recurrent, high-level copy number gain (inferred copy number > 2.14) and 20 regions of recurrent copy number loss (inferred copy number < 1.86)
Project description:We analyzed active enhancers of nasopharyngeal epithelial cell lines and nasopharyngeal carcinoma cell lines by performing ChIP-seq on H3K4me1, H3K4me3, and H3K27ac.
