Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_bc_mapping]

ABSTRACT: A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_bc_mapping]

PROVIDER: PRJNA1149079 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis

Project description:A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis

| PRJNA1149070 | ENA

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [fiveprime_end_seq]

Project description:A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [fiveprime_end_seq]

| PRJNA1149056 | ENA

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [threeprime_end_seq]

Project description:A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [threeprime_end_seq]

| PRJNA1149060 | ENA

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [rend_seq]

Project description:A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [rend_seq]

| PRJNA1149061 | ENA

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_quant]

Project description:A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_quant]

| PRJNA1149075 | ENA

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [fiveprime_end_seq]

Project description:RNA endonucleases are the rate-limiting initiator of decay for many bacterial mRNAs. However, the positions of cleavage and their sequence determinants remain elusive even for the well-studied Bacillus subtilis. Here we present two complementary approaches – transcriptome-wide mapping of endoribonucleolytic activity and deep mutational scanning of RNA cleavage sites – that reveal distinct rules governing the specificity among B. subtilis endoribonucleases. Detection of RNA terminal nucleotides in both 5′- and 3′-exonuclease-deficient cells revealed >103 putative endonucleolytic cleavage sites with single-nucleotide resolution. We found a surprisingly weak consensus for RNase Y targets, a contrastingly strong primary sequence motif for EndoA targets, and long-range intramolecular secondary structures for RNase III targets. Deep mutational analysis of RNase Y cleavage sites showed that the specificity is governed by many disjointed sequence features, each with mild contributions. Our results highlight the delocalized nature of mRNA stability determinants and provide a strategy for elucidating endoribonuclease specificity in vivo.

2025-01-15 | GSE275074 | GEO

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_quant]

2025-01-15 | GSE275081 | GEO

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [mpra_bc_mapping]

2025-01-15 | GSE275080 | GEO

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [threeprime_end_seq]

2025-01-15 | GSE275077 | GEO

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis [rend_seq]

2025-01-15 | GSE275076 | GEO