Project description:Root and leave samples of 4 different apple genotypes were investigated in order to analyse the gene expression after infection with Apple Replant Disease (ARD). All genotypes were cultivated in ARD-infected soil and gamma-irradiated (disinfected) soil in the greenhouse for 7 days. The ARD soil originated from two different orchards representing two different soil compositions. After 7 days root tissue was collected from each plant and used for the subsequent gene expression analysis. This work was part of the project BonaRes-ORDIAmur funded by the German Federal Ministry of Research and Education within the frame of the program BonaRes (grant no. 031B0025B). It was also funded by the German Research Foundation (DFG) via the research training group GRK1798 "Signaling at the Plant-Soil Interface" and a grant to BL and LB (BE 1174/19-1).
Project description:Transcriptional profiling of various apple (Malus x domestica Borkh) organ systems using probes complementary to both sense and anti-sense transcripts. Eight apple organs/samples. Biological replicates: 2 for each sample, independently grown and harvested.
Project description:Closed terminal buds of apple trees (Malus x domestica Borkh, Royal Gala and Castel Gala varieties) grown in commercial orchards were harvested during autumn and winter and exposed to cold treatments
2015-04-06 | GSE64717 | GEO
Project description:Microbial community of apple rhizosphere from different replant soils
Project description:In order to understand the role of phloems of apple dwarfing rootstocks,and investigated the expression differences of dwarfing and vigorous apple stocks in the bud break stage, The phloem tissue at bud break stage(0 DABB(days after buds break) of three apple rootstocks including A1d（a partial GA insensitive mutant of Malus hupehensis ),WT Malus hupehensis and were QZ1(a hybrid of Malus hupensis and a Cylindrical apple variety) were sampled and underwent RNA-Seq analysis.
Project description:The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to 4 are allergens present in a variety of different isoforms in apples, with Mal d1 and Mal d3 being the main allergens. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. We revealed the expression of at least 21 Mal d1 isoforms while two Mal d3 and two Mal d4 proteins were found among the genotypes. The allergenic potential of the Mal d isoforms was characterized by comparing isoform abundance with allergenic scores of genotypes (from oral challenge tests). The Mal d peptides detected by comparative analysis presumably have different IgE binding properties and could therefore be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars.
Project description:The main objective of this analysis was to sequence the epigenome of two apple (Malus domestica) doubled haploid 'Golden Delicious' fruits (GDDH13 and GDDH18). Our secondary objective was to identify differentially methylated regions between DNA purified from the GDDH13 genotype and the GDDH18 genotypes at two developmental stages.
2017-04-27 | GSE93950 | GEO
Project description:Molecular barcoding reveals bacteria of the Streptomycetes as important members of root endophytes of apple plants (Malus domestica M26) grown in soils affected by apple replant disease
Project description:in order to understand the role of phloems of apple dwarfing rootstocks,and investigated the expression differences of dwarfing and vigorous apple stocks in the phloem tissue at active growing stage. The phloem tissue at active growing stage(60 DABB(days after buds break) of three apple dwarfing rootstocks including M9,B9,A1d（a partial GA insensitive mutant of Malus hupensis）and two vigorous apple rootstock PYTC ( WT of Malus hupensis) and M. sylvestris were sampled and underwent RNA-Seq analysis.
