Project description:To investigate the impact of the reproductive cycle on adipose tissue, we analyzed transcriptome profiling across the entire estrous cycle in the adipose tissue of female mice.
Project description:Adipose tissue is crucial for energy storage and release, ensuring energy homeostasis within the body. Disturbances in the physiology of adipose tissue have been associated with various health disorders, such as obesity and diabetes. The reproductive cycle represents a fundamental biological pattern in female physiology. Although previous research has highlighted the substantial regulatory influence of ovarian hormones on adipose tissue, our understanding of the comprehensive changes in adipose tissue throughout the reproductive cycle remains limited. In this study, we examined the transcriptomic profile of female mouse-adipose tissue across their complete estrous cycles. The findings provided detailed descriptions of the datasets generated, including information on data collection, processing, and quality control. The study also demonstrated the robustness of these data through various validation steps. These findings serve as crucial resources for investigating the role of estrous cycle rhythmicity in important adipose tissue processes in the future.
Project description:It is well known that host-microbes and immunity interactions are influenced by dietary patterns, as well as daily environmental light-dark (LD) cycles that entrain circadian rhythms in the host. Emerging data has highlighted the importance of diet patterns and timing on the interaction among circadian rhythms, gut microbiome, and immunity, however, their impacts on LD cycles are less reported. Therefore, we aim to study how LD cycles regulate the homeostatic crosstalk between gut microbiome, hypothalamic and hepatic circadian clock oscillations and immunity. We hypothesized that different environmental LD cycles: (1) constant darkness, LD0/24; (2) short light, LD8/16; (3) normal LD cycle, LD12/12; (4) long light, LD16/8; and (5) constant light, LD24/0, may affect immunity and metabolism to varying degrees. Therefore, 240 mice were managed with chow diets (CD) and antibiotics treatments (ABX) under five different LD cycles for 42 days. The liver (LIV), hypothalamus (HYP), inguinal white adipose tissue (iWAT), ileum epithelium (ILE), colon epithelium (COL), jejunum epithelium (JEJ), cecum epithelium (CEC), spleen (SPL), mammary gland (MAG), and thymus gland (THY) tissues were obtained for studying their impacts immunity using RNA-Seq data.
Project description:Transcriptional profiling of transgenic mice specifically expressing amphiregulin in white adipose tissues. The objective of this study is to explore gene expression profiles of adipose tissues in response to amphiregulin overexpression.
Project description:To investigate the role of somatosensory innervation of adipose tissues, we unilaterally ablate sensory innervation of adipose tissues, and profiled the gene expression in the inguinal adipose tissues 4 weeks after sensory ablation surgery.
Project description:Gene expression profiling of supraclavicular brown, interscapular brown, inguinal white, and epididymal white adipose tissues from C57BL/6 male mice was performed by RNA-sequencing.