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ABSTRACT: Analysis of miRNAs in winter Brassica rapa under drought stress

PROVIDER: PRJNA1150664 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30335140.fastq.gz	Fastqsanger.gz
	SRR30335141.fastq.gz	Fastqsanger.gz
	SRR30335142.fastq.gz	Fastqsanger.gz
	SRR30335143.fastq.gz	Fastqsanger.gz
	SRR30335144.fastq.gz	Fastqsanger.gz

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Project description:Transcriptomic analysis of winter Brassica rapa L. under drought stress

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iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to cold stress in two Winter Turnip Rape (Brassica rapa L.), part 2

Project description:Winter turnip rape (Brassica rapa L.) is a valuable ecologically beneficial oil crop that is produced mainly for its ability of conserving soil and water in winter and spring and its high quality edible oil in northwestern China. However, coldness and extremely low temperature negatively affects the growth and development of winter turnip rape, resulting in failure to overwinter and production in northwestern China. ‘Longyou 7’(Brassica rapa L.) and ‘Tianyou 4’ (Brassica rapa L.) are closely related plant species, but their cold tolerances are different. ‘Longyou 7’ is a cold-tolerant cultivar, ‘Tianyou 4’is a cold-sensitive cultivar. In this study, we used iTRAQ-based proteomics to compare quantitative changes in the proteome of two winter turnip rape leaves and roots in response to cold stress to elucidate the possible molecular mechanism underlying the ability of ‘Longyou 7’ to adapt to cold stress.

2019-11-12 | PXD008195 | Pride

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to cold stress in two Winter Turnip Rape (Brassica rapa L.), part 1

2019-11-12 | PXD008194 | Pride

Gene expression profiling of drought tolerant and sensitive Brassica rapa accessions under control and osmotic stress conditions

Project description:In this study genome-wide gene expression profiling was used to analyze mechanisms of drought tolerance in Brassica rapa. Using an Illumina Mi-Seq platform we sequenced RNA from shoot tissues of drought tolerant and drought sensitive B. rapa genotypes in control conditions and after application of osmotic stress. Differentially expressed genes between the different conditions and genotypes were used to identify drought relevant gene networks.

2017-08-31 | GSE73963 | GEO

Project description:Transcriptomic analysis of winter Brassica rapa L. under saline-alkali stress

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Project description:MeRIP-seq analysis of winter Brassica rapa under low temperature stress

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Identification and characterization of miRNAs in oilseed rape (Brassica napus) responsive to the pathogenic fungus Verticillium longisporum infection using Brassica AA (B. rapa) and CC (B. oleracea) as reference genomes

Project description:we deep-sequenced two small RNA libraries made from V. longisporum infected/non-infected roots and employed Brassica rapa and Brassica oleracea genomes as reference for miRNA prediction and characterization as well. We identified 893 B. napus miRNAs representing 360 conserved and 533 novel miRNAs, and mapped 429 and 464 miRNAs to AA and CC genomes, respectively. Among them, 62 miRNAs were responsive to the V. longisporum infection.

2014-10-06 | GSE58322 | GEO

Expression profile of trace metal element responsive genes in Brassica rapa

Project description:Purpose: Zinc deficiency (ZnD) and iron deficiency (FeD), excess Zn (ZnE) and cadmium exposure (CdE) are major environmental problems for crop cultivation. Methods: Applying Tag-Seq technology to leaves of Brassica rapa grown under FeD, ZnD, ZnE or CdE conditions, with normal conditions as a control, we examined global gene expression changes and compared the expression patterns of multiple paralogs. Results: We identified 812, 543, 331 and 447 differentially expressed genes under FeD, ZnD, ZnE and CdE conditions, respectively, in B. rapa leaves.Further analysis revealed that genes associated with Zn, Fe and Cd responses tended to be over-retained in the B. rapa genome. Most of these multiple-copy genes showed the same direction of expression change under stress conditions. Conclusion: We conclude that the duplicated genes involved in trace element responses in B. rapa are functionally redundant, making the regulatory network more complex in B. rapa than in Arabidopsis thaliana.

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Turnip succinylation modification

Project description:Quantitative succinyl-proteome profiling of turnip (Brassica rapa var. rapa) in response to cadmium stress.

2022-08-11 | PXD028098 | Pride

Network analysis identifies temporal regulation of transcriptomic and physiological responses to early drought perception in Brassica rapa.

Project description:The temporal control of gene expression leads to time of day effects on metabolism and physiology. To identify early transcript level changes occurring in the Brassica rapa oil-type variety R500 during initial drought perception we performed an RNA-seq time course experiment. Leaf tissue was collected from well-watered and drought treated 18 day old plants grown under 14h light at 22°C/10h dark at 18°C every 4h over 2 days with 2 biological replicates at every time point. We collected physiology measurements at every time point and observed dynamic time of day changes in photosynthetic rate, stomatal conductance, non-structural carbohydrates and PSII efficiency. Using a co-expression network approach, phase dependent gene modules were correlated with the physiology data to reveal temporally regulated drought responses. This study provides insight into the transcriptome level responses occurring in the plant in the early stages of drought perception before visible signs of stress occur.

2017-08-19 | GSE90841 | GEO

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