Project description:The cadmium-resistant Cupriavidus sp. E324 strain has been previously shown to have a high potential for use in cadmium (Cd) remediation, due to its high capacity for cadmium bioaccumulation. According to the comparative genomic analysis, the E324 strain was most closely related to C. nantongensis X1 T , indicating that the E324 strain should be re-identified as C. nantongensis. To unravel the Cd tolerance mechanisms of C. nantongensis E324, the transcriptional response of this strain to Cd stress was assessed using RNA-seq-based transcriptome analysis, followed by validation through qRT- PCR. The results showed that the upregulated Differentially Expressed Genes (DEGs) were significantly enriched in categories related to metal binding and transport, phosphate transport, and oxidative stress response. Consistently, we observed significant increases in both the cell wall and intracellular contents of certain essential metals (Cu, Fe, Mn, and Zn) upon Cd exposure). Among these, only the Zn pretreatment resulting in high Zn accumulation in the cell walls could enhance bacterial growth under Cd stress conditions through its role in inhibiting Cd accumulation. Additionally, the promotion of catalase activity and glutathione metabolism upon Cd exposure to cope with Cd-induced oxidative stress was demonstrated. Meanwhile, the upregulation of phosphate transport-related genes upon Cd treatment seems to be the bacterial response to Cd-induced phosphate depletion. Altogether, our findings suggest that these adaptive responses are critical mechanisms contributing to increased Cd tolerance in C. nantongensis E324 strain via the enhancement of metal-chelating and antioxidant capacities of the cells.

2024-09-03 | GSE272856 | GEO

Genomic and transcriptomic analyses reveal insights into cadmium resistance mechanisms of Cupriavidus nantongensis E324

Project description:Genomic and transcriptomic analyses reveal insights into cadmium resistance mechanisms of Cupriavidus nantongensis E324

| PRJNA1139126 | ENA

Metal resistant bacterium Cupriavidus metallidurans CH34

Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress;

2014-09-13 | GSE24094 | GEO

Stress response of Cupriavidus metallidurans strains

Project description:Response of Cupriavidus metallidurans CH34, AE104 and delta zupT mutant to Zn/EDTA stress In this study 3 different treatments were performed to acquire expression profiles of the total genome of Cupriavidus metallidurans

2014-12-16 | E-GEOD-64196 | biostudies-arrayexpress

Cupriavidus metallidurans strain:ZM16

Project description:Cupriavidus metallidurans strain:ZM16 Genome sequencing

| PRJNA794029 | ENA

Cupriavidus gilardii strain:QJ1

Project description:Cupriavidus gilardii strain:QJ1 Genome sequencing

| PRJNA846986 | ENA

Cupriavidus cauae strain:PHS1

Project description:Cupriavidus cauae strain:PHS1 Genome sequencing

| PRJNA749683 | ENA