Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exploration of miRNAs in diabetic foot ulcers of mice

ABSTRACT: Exploration of miRNAs in diabetic foot ulcers of mice

PROVIDER: PRJNA1153268 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30426963.fastq.gz	Fastqsanger.gz
	SRR30426964.fastq.gz	Fastqsanger.gz
	SRR30426965.fastq.gz	Fastqsanger.gz
	SRR30426966.fastq.gz	Fastqsanger.gz
	SRR30426967.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Exploration of miRNAs in diabetic foot ulcers of mice

Project description:To investigate the expression of miRNAs in C57 diabetic ulcer mice by Small RNA sequencing

2024-08-31 | GSE275847 | GEO

Single Cell Transcriptomic Landscape of Diabetic Foot Ulcers

Project description:Diabetic foot ulcers (DFUs) are a devastating complication of diabetes. In order to identify systemic and local factors associated with DFU healing, we examined the cellular landscape of DFUs by single-cell RNA-seq analysis of foot and forearm skin specimens, as well as PBMC samples, from 10 non-diabetic subjects, and 17 diabetic patients, 11 with, and 6 without DFU. Our analysis shows enrichment of a unique inflammatory fibroblast population in DFU patients with healing wounds. The patients with healing DFUs also depicted enrichment of macrophages with M1 polarization, as opposed to more M2 macrophages in non-healing wounds. These findings were verified using Immunohistochemistry and Spatial Transcriptomics.

2021-11-01 | GSE165816 | GEO

Circular RNA expression profiling in diabetic foot ulcers and human normal acute wounds

Project description:Circular RNA (circRNA) microarray analysis was performed to examine the expression profiles of circRNAs in diabetic foot ulcers (DFU) and in human excisional skin wounds 7 days after injury.

2019-10-01 | GSE114248 | GEO

Spatial transcriptomics of healing and non-healing diabetic foot ulcers

Project description:Diabetic foot ulcers (DFUs) are a devastating complication of diabetes. To better understand the molecular mechanisms and cell types implicated in DFU healing, we used NanoString’s GeoMx Digital Spatial profiling platform on DFU tissue sections and compared gene expression of areas within the same ulcer as well as between patients who in 12 weeks following surgery healed their DFU (Healers, N=2) vs those who did not (Non-Healers, N=2).

2021-10-28 | GSE166120 | GEO

Characterization of Diabetic and Non-Diabetic Foot Ulcers Using Single-Cell RNA-Sequencing

Project description:We examined the feasibility of single cell RNA sequencing (scRNA-seq) analysis to evaluate human chronic wound samples acquired in the clinic, subjected to prolonged cold ischemia time, and processed without FACS sorting. Wound tissue from human diabetic and non-diabetic plantar foot ulcers were evaluated using an optimized 10X Genomics scRNA-seq platform and analyzed using a modified data pipeline designed for low-yield specimens. Cell subtypes were identified informatically and their distributions and transcriptional programs were compared between diabetic and non-diabetic tissue. 139,000 diabetic and non-diabetic wound cells were delivered for 10X capture after either 90 or 180 min of cold ischemia time. cDNA library concentrations were 858.7 and 364.7 pg/µL, respectively, prior to sequencing. Among all barcoded fragments, we found that 83.5% successfully aligned to the human transcriptome and 68% met the minimum cell viability threshold. The average mitochondrial mRNA fraction was 8.5% for diabetic cells and 6.6% for non-diabetic cells, correlating with differences in cold ischemia time. A total of 384 individual cells were of sufficient quality for subsequent analyses; from this cell pool, we identified transcriptionally-distinct cell clusters whose gene expression profiles corresponded to fibroblasts, keratinocytes, neutrophils, monocytes, and endothelial cells. Fibroblast subpopulations with differing fibrotic potentials were identified, and their distributions were found to be altered in diabetic vs. non-diabetic cells.

2023-11-25 | GSE248247 | GEO

Gene expression profile as single cell level of chronic diabetic foot ulcers and chronic non-diabetic foot ulcer

Project description:We investigate the changes between diabetic and non-diabetic wound healing process in chronic foot ulcer.

2023-02-01 | GSE223964 | GEO

Single Cell Analysis of Gene expression profile as single cell level of chronic diabetic foot ulcers and chronic non-diabetic foot ulcer Foot Wounds

Project description:We investigate the changes between diabetic and non-diabetic wound healing process in chronic foot ulcer.

2023-10-24 | GSE245703 | GEO

Spatial transcriptomics of healing and non-healing diabetic foot ulcers

Project description:Spatial transcriptomics of healing and non-healing diabetic foot ulcers

| PRJNA699294 | ENA

Characterization of Diabetic and Non-Diabetic Foot Ulcers Using Single-Cell RNA-Sequencing

Project description:Characterization of Diabetic and Non-Diabetic Foot Ulcers Using Single-Cell RNA-Sequencing