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NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [multiome]

ABSTRACT: NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [multiome]

PROVIDER: PRJNA1153539 | ENA |

REPOSITORIES: ENA

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NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [multiome]

Project description:Testicular fetal Leydig cells are specialized for androgen production during embryogenesis. Testosterone is essential for regulating sex differentiation, spermatogenesis, and fertility. Deficiencies in Leydig cell differentiation can lead to various disorders of sex development and male reproductive conditions, such as ambiguous genitalia, hypospadias, cryptorchidism, and infertility. Understanding the differentiation of fetal Leydig cells is essential for comprehending male sexual differentiation, reproductive health, and fertility. Fetal Leydig cells originate from proliferating progenitor cells in the gonadal interstitium, marked by genes like Arx, Pdgfra, Tcf21, Wnt5a, and Nr2f2. However, the precise mechanisms governing the transition from interstitial cells to Leydig cells remain elusive. Through integrated approaches involving animal models and multiomics, we have demonstrated that fetal Leydig cells originate from a Nr2f2 positive non-steroidogenic interstitial cell population. NR2F2 promotes progenitor cell fate while suppressing Leydig cell differentiation. Moreover, embryonic deletion of Nr2f2 in mouse testes resulted in disorders of sex development, including reduced testicular size, Leydig cell hypoplasia, cryptorchidism, and hypospadias. Collectively, our findings highlight the critical role of Nr2f2 in orchestrating the transition from interstitial cells to Leydig cells during testicular development.

2025-03-24 | GSE275901 | GEO

NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [ChIP-Seq]

Project description:Testicular fetal Leydig cells are specialized for androgen production during embryogenesis. Testosterone is essential for regulating sex differentiation, spermatogenesis, and fertility. Deficiencies in Leydig cell differentiation can lead to various disorders of sex development and male reproductive conditions, such as ambiguous genitalia, hypospadias, cryptorchidism, and infertility. Understanding the differentiation of fetal Leydig cells is essential for comprehending male sexual differentiation, reproductive health, and fertility. Fetal Leydig cells originate from proliferating progenitor cells in the gonadal interstitium, marked by genes like Arx, Pdgfra, Tcf21, Wnt5a, and Nr2f2 (COUP-TFII). However, the precise mechanisms governing the transition from interstitial cells to Leydig cells remain elusive. Through integrated approaches involving animal models and multiomics, we have demonstrated that fetal Leydig cells originate from a COUP-TFII positive non-steroidogenic interstitial cell population. COUP-TFII promotes progenitor cell fate while suppressing Leydig cell differentiation. Moreover, embryonic deletion of COUP-TFII in mouse testes resulted in disorders of sex development, including reduced testicular size, Leydig cell hypoplasia, cryptorchidism, and hypospadias. Collectively, our findings highlight the critical role of COUP-TFII in orchestrating the transition from interstitial cells to Leydig cells during testicular development.

2025-03-24 | GSE275900 | GEO

NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [RNA-Seq]

2025-03-24 | GSE275902 | GEO

NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [ChIP-Seq]

Project description:NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [ChIP-Seq]

| PRJNA1153544 | ENA

NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [RNA-Seq]

Project description:NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [RNA-Seq]

| PRJNA1153540 | ENA

Expression data from NR2F2(COUP-TFII) depleted mouse MA-10 Leydig cells

Project description:Expression data from NR2F2(COUP-TFII) depleted mouse MA-10 Leydig cells

| PRJNA685545 | ENA

Expression data from NR2F2(COUP-TFII) depleted mouse MA-10 Leydig cells

Project description:In males, Leydig cells are the main producers of testosterone and insulin-like 3, hormones which are both essential for sex differentiation and reproductive functions. Nuclear receptor chicken ovalbumin upstream promoter-transcription factors II (COUP-TFII) is expressed in the cells committed to give rise to the fully functional steroidogenic adult Leydig cells and has a major role in their function and differentiation. Up to date, only handful of COUP-TFII gene targets have been reported. A transcriptomic approach was used to identify additional genes affected by depletion of COUP-TFII in mouse MA-10 Leydig cell line.

2021-07-01 | GSE163283 | GEO

Purification and transcriptomic analysis of mouse fetal Leydig cells reveals candidate genes for disorders of sex development

Project description:To examine the transcriptome of early testicular somatic cells during gonadogenesis at 12.5dpc RNA sequencing (RNA-Seq) was performed on murine primary testicular cell lineages isolated from the Sf1-eGFP line by FACS. The three main somatic cell lineages of the testis were isolated: the Sertoli cells which direct male development; the fetal Leydig cells (FLCs) that produce steroid hormones and virilise the XY individual and a heterogenous population of interstitial cells, some of which give rise to the adult Leydig cells (ALCs). This dataset provides a platform for exploring the biology of FLCs and understanding the role of these cells in testicular development and masculinization of the embryo, and a basis for targeted studies designed to identify causes of idiopathic XY DSD. RNA-Seq of 3 enriched cell populations from 12.5dpc mouse gonad (Sertoli cells, Leydig cells and Interstitial cells isolated by FACS-sorting) on an Illumina HiSeq 1500, in triplicate.

2015-05-21 | E-GEOD-65498 | biostudies-arrayexpress

Purification and transcriptomic analysis of mouse fetal Leydig cells reveals candidate genes for disorders of sex development

2015-05-21 | GSE65498 | GEO

NR2F2 study

Project description:NR2F2 study

| PRJNA548079 | ENA