Project description:PICKLE (PKL), a Chromodomain Helicase DNA binding domain type 3-type (CHD3) chromatin remodeler, noted for an embryonic structure called pickle root in primary root tip in pkl mutant, has been studied for decades. we obtained a comprehensive genome occupancy of PKL by Chromatin immunoprecipitation-sequencing (ChIP-seq), and found PKL co-occupy with the major repressors of seed maturation program, VIVIPAROUS1/ABI3-LIKE1/2 (VAL1/2) in genome. Furthermore, PKL physically interacts with VAL1/2 in vivo and phenotype and transcriptome data indicated that PKL and VAL1/2 function in a common pathway. Moreover, ChIP-seq and ChIP-qPCR results showed that VAL1/2 are necessary for the recruitment of PKL to co-target genes
Project description:4 weeks old rooted plantlets (P. tremula × tremuloides) of wildtype (T89), PICKLE:RNAi (line 417-4 and 417-17) and FDL2:RNAi (line 510-12 and 510-18) were potted in soil in 1.5 l pots and were kept in a glass chamber. The plants were grown for eight weeks and well irrigated before the treatment started. Well-watered and drought stress treatments were applied. Woody samples were collected after 4 weeks of treatment for RNA extraction and RNA sequencing.
