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Inositol polyphosphates regulate resilient mechanisms in the green alga Chlamydomonas reinhardtii to adapt to extreme nutrient conditions

ABSTRACT: Inositol polyphosphates regulate resilient mechanisms in the green alga Chlamydomonas reinhardtii to adapt to extreme nutrient conditions

PROVIDER: PRJNA1156089 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30527336.fastq.gz	Fastqsanger.gz
	SRR30527337.fastq.gz	Fastqsanger.gz
	SRR30527338.fastq.gz	Fastqsanger.gz
	SRR30527339.fastq.gz	Fastqsanger.gz
	SRR30527340.fastq.gz	Fastqsanger.gz

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Inositol polyphosphates regulate resilient mechanisms in the green alga Chlamydomonas reinhardtii to adapt to extreme nutrient conditions

Project description:In the actual context of climate changing environments, photosynthetic organisms need to adapt to more extreme conditions. Microalgae can be excellent organisms to understand molecular mechanisms that activate survival mechanisms under stress. Chlamydomonas reinhardtii signaling mutants are extremely useful to decipher which strategies they use to cope with changeable environments. In this study, we conducted prolonged starvation in wild type and vip1-1 Chlamydomonas cells. The mutant vip1-1 has an altered profile of pyroinositol polyphosphates (PP-InsPs) which are signaling molecules present in all eukaryotes. These molecules have been connected to P signaling in other organisms including plants but their implications in other nutrient signaling is still under evaluation. After prolonged starvation, WT and vip1-1 showed important differences in the levels of chlorophyll and PSII activity. We also performed a metabolomic analysis under these conditions and found an overall decrease in different organic compounds such as amino acids including arginine and its precursors and tryptophan which is considered as a signaling molecule itself in plants. In addition, we observed significant differences in RNA levels of genes related to nitrogen assimilation that are under the control of NIT2 transcription factor. Overall our data indicate an important role of PP-InsPs in the regulation of nutrient starvation especially regarding N assimilation and C distribution. These data are of great importance for the generation of resilient strains to be used in open ponds and high capacity bioreactors.

2024-09-17 | GSE276249 | GEO

Transcriptome analysis of cryptococcal IPK mutants

Project description:In eukaryotes, inositol polyphosphates perform essential metabolic and signaling functions. Using human fungal pathogen Cryptococcus neoformans as a model, we created mutants in three inositol polyphosphates kinases: Arg1, Ipk1 and Kcs1. Each of the mutants produces a unique repertoire of inositol polyphosphates, different from the wild type strain. Comparative phenotypic and transcriptome analyses of wild type and mutant strains indicates that inositol polyphosphate PP-IP5 (IP7) is the key regulator of gene expression, fitness and virulence in C. neoformans.

2016-03-03 | GSE78824 | GEO

Differentiation and homeostasis of effector Treg cells are regulated by inositol polyphosphates modulating Ca2+ influx

Project description:Differentiation and homeostasis of effector Treg cells are regulated by inositol polyphosphates modulating Ca2+ influx

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Transcriptome analysis of cryptococcal IPK mutants

2016-03-03 | E-GEOD-78824 | biostudies-arrayexpress

Differentiation and homeostasis of effector Treg cells are regulated by inositol polyphosphates modulating Ca2+ influx

Project description:Activated Foxp3+ regulatory T (Treg) cells differentiate into effector Treg (eTreg) cells to maintain peripheral immune homeostasis and tolerance. T cell receptor (TCR)-mediated induction and regulation of store-operated Ca2+ entry (SOCE) is essential for eTreg cell differentiation and function. However, SOCE regulation in Treg cells remains unclear. Here we show that inositol polyphosphate multikinase (IPMK), which generates inositol tetrakisphosphate and inositol pentakisphosphate, is a pivotal regulator of Treg cell differentiation downstream of TCR signaling. IPMK is highly expressed in TCR-stimulated Treg cells and promotes a TCR-induced Treg cell program. IPMK-deficient Treg cells display aberrant T cell activation and impaired differentiation into RORγt+ Treg cells, and tissue-resident Treg cells. Mechanistically, IPMK controls the generation of higher-order inositol phosphates, thereby promoting Ca2+ mobilization and Treg cell effector functions. Our findings identify IPMK as a critical regulator of TCR-mediated Ca2+ influx and highlight the importance of IPMK in Treg cell-mediated immune homeostasis.

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Effect of polyphosphates on osteoblast mineralization

Project description:Rat osteoblasts ROB17/2.8 cells were cultured in the presence of high (1mg/ml) or low (0.01mg/ml) concentration of polyphosphates.

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Effect of polyphosphates on osteoblast mineralization

Project description:Rat osteoblasts ROB17/2.8 cells were cultured in the presence of high (1mg/ml) or low (0.01mg/ml) concentration of polyphosphates. Poly(P) induced gene expression in rat osteoblast ROS17/2.8 cells was measured after the treatment with high (1mg/ml) or low (0.01mg/ml) for 24 h.

2016-03-15 | E-GEOD-79176 | biostudies-arrayexpress

A resilient mutualistic interaction between cucumber mosaic virus and its natural host Arabidopsis halleri to adapt to an environmental change

Project description:A pseudometallophyte Arabidopsis halleri is frequently found to be infected with cucumber mosaic virus (CMV) in its natural habitat. The purpose of this study is to elucidate the effect of CMV infection on its natural hosts. The CMV(Ho) strain isolated from A. halleri was inoculated into clonal A. halleri plants that were obtained from runners of mother plants, and the pathosystem consisting of CMV(Ho) and its natural host A. halleri was established.In low heavy metal environment, the CMV(Ho) infection caused growth retardation in the above-ground part (stems and leaves) of host plants, and thereby conferred strong drought tolerance on host plants. On the other hand, in high heavy metal environment, which simulates a natural habitat for A halleri, the CMV(Ho) infection did not cause any symptoms to host plants and conferred mild drought tolerance. And the result of transcriptome analysis suggests that CMV(Ho) is recognized as a symbiont rather than a pathogen by its host plant. These results indicate a resilient mutualistic interaction between CMV(Ho) and its natural host A. halleri to adapt to an environmental change.

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Regulation of chromatin remodeling by inositol polyphosphates.

Project description:Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations in ARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling of PHO5 promoter chromatin is impaired, and the adenosine triphosphate-dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.

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A resilient mutualistic interaction between cucumber mosaic virus and its natural host Arabidopsis halleri to adapt to an environmental change

Project description:A resilient mutualistic interaction between cucumber mosaic virus and its natural host Arabidopsis halleri to adapt to an environmental change

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