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Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter

ABSTRACT: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter

PROVIDER: PRJNA1158716 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30610032_1.fastq.gz	Fastqsanger.gz
	SRR30610032_2.fastq.gz	Fastqsanger.gz
	SRR30610033_1.fastq.gz	Fastqsanger.gz
	SRR30610033_2.fastq.gz	Fastqsanger.gz
	SRR30610034_1.fastq.gz	Fastqsanger.gz

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Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter

Project description:Influenza A virus encodes promoters in both the sense and antisense orientations. These support the generation of new genomes, antigenomes, and mRNA transcripts. Characterization of the influenza promoters using minimal replication assays—transfections with viral polymerase, nucleoprotein, and a genomic template—defined their sequence as 13nt at the 5’ end of the viral genomic RNA (U13) and 12nt at the 3’ end (U12).Other than a single position the U12 and U13 sequences are identical between all eight RNA molecules that comprise the segmented influenza genome.Nevertheless, each segment can exhibit different transcriptional dynamics despite possessing identical promoters.Minimal replication assays lack the influenza protein NS2, which can modulate transcription and replication differentially between influenza segments.This suggests that NS2 expression may redefine the influenza A virus promoter.In this work we assess how internal regions of sequence in two genomic segments, HA and PB1, may contribute to NS2-dependent replication as well as map such interactions down to individual nucleotides in PB1. We find that the expression of NS2 significantly alters sequence requirements for efficient replication beyond the identical U12 and U13 sequence, providing a mechanism for the divergent replication and transcription dynamics across the influenza A virus genome.

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