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ABSTRACT: Transcriptome sequencing of Fusarium solani

PROVIDER: PRJNA1160924 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30664868_1.fastq.gz	Fastqsanger.gz
	SRR30664868_2.fastq.gz	Fastqsanger.gz
	SRR30664869_1.fastq.gz	Fastqsanger.gz
	SRR30664869_2.fastq.gz	Fastqsanger.gz
	SRR30664870_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Fusarium solani (NK-NH1) before and after inhibition of volatile organic compounds produced by Xenorhabdus bovienii

Project description:In order to study the inhibition mechanism of volatile organic compounds produced by Xenorhabdus bovienii on Fusarium solani (NK-NH1), we selected the inhibited and uninhibited Fusarium solani mycelia for transcriptome sequencing, and tried to find the corresponding inhibition mechanism at the gene level.

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New insights into antifungal resistance mechanisms in Fusarium species revealed by high throughput sequencing

Project description:Fusarium spp. are fungal pathogens of humans and plants. Fusarium oxysporum and Fusarium solani are important species isolated from infections such as onychomycosis, fungal keratitis, invasive infections, and disseminated diseases. These pathologies have a very difficult therapeutic management and poor therapeutic responses, especially in patients with disseminated infection. Little information is available regarding the molecular mechanisms responsible for antifungal resistance in these fungi. methods: In this study, we performed a quantitative analysis of the transcriptional profile of F. oxysporum and F. solani, challenged with amphotericin B (AMB) and posaconazole (PSC) using RNA-seq. Quantitative real-time reverse transcription PCR (qRT-PCR) was used to validate the results results: Several genes related to mechanisms of antifungal resistance such as efflux pumps, ergosterol pathway synthesis, and responses to oxidative stress were found. Genes such as ERG11, ERG5, the Major Facilitator Superfamily (MFS), thioredoxin, and different dehydrogenase genes may explain the reduced susceptibility of Fusarium spp. against azoles and the possible mechanisms that may play an important role in induced resistance against polyenes. conclusions: Important differences in the levels of transcriptional expression were found between F. oxysporum and F. solani exposed to the two different antifungal treatments. Knowledge on the gene expression profiles and gene regulatory networks in Fusarium spp. during exposure to antifungal compounds, may help to identify possible molecular targets for the development of novel, better, and more specific therapeutic compounds. profile transcriptional of Fusarium spp changed to antifungal treatments in vitro

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