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LSK immunophenotype hematopoietic stem cells in Dapp1 knockout mice

ABSTRACT: LSK immunophenotype hematopoietic stem cells in Dapp1 knockout mice

PROVIDER: PRJNA1161661 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30677581_1.fastq.gz	Fastqsanger.gz
	SRR30677581_2.fastq.gz	Fastqsanger.gz
	SRR30677582_1.fastq.gz	Fastqsanger.gz
	SRR30677582_2.fastq.gz	Fastqsanger.gz
	SRR30677583_1.fastq.gz	Fastqsanger.gz

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LSK immunophenotype hematopoietic stem cells in Dapp1 knockout mice

Project description:To investigate the impact of perturbing key differentiation driver genes on hematopoietic stem cell fate decision, we employed a Dapp1 gene knockout approach. This allowed us to observe changes in the subpopulations of hematopoietic stem cells at the LSK level in mice. Additionally, we assessed alterations in their fate decision and lineage differentiation tendencies

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LSK immunophenotype hematopoietic stem cells after irradiation at day 2

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LSK immunophenotype hematopoietic stem cells after irradiation at day points in 3 weeks

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LSK immunophenotype hematopoietic stem cells after irradiation at day points in 3 weeks

Project description:In this study, we aimed to investigate the differentiation dynamics of LSK (Lin-Sca1+c-Kit+) cells following radiation injury. LSK cells, which are a critical component of the hematopoietic stem and progenitor cell population, play a vital role in the regeneration and repair of the hematopoietic system after damage. By utilizing these cells, we sought to understand the mechanisms underlying their response to radiation, focusing on their capacity for self-renewal and differentiation into various blood cell lineages.

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Project description:Gene expression of LSK (lin-Sca-Kit+) hematopoietic stem cells from wild type mice was compared with LSK from Cebpa knock-in mutant mice (K/K, K/L, and L/L mutants). Fetal liver cells for each genotype were competitively transplanted into irradiant recipients. Donor-derived LSK cells were isolated by FACS sorting of recipient bone marrow. 3 biological replicates of each were generated and expression profiles were determined by hybridization to Affymetrix Moe430_2 arrays.

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Expression data from LSK cells from mice with knock-out of Ezh1 versus LSK control

Project description:Ezh1 is a protein member of PRC2. Ezh1 has been described as a functional repressor gene, such as its homologous Ezh2. We are investigating the role of Ezh1 in hematopoietic stem cells, aging, self-renewal and differentiation. We used microarrays to detail the global program of gene expression in LSK cells from mice with knocked-down expression of Ezh1. LSK cells from EZH1 knockout and control mice were marked and isolated by fluorescence-activated cell sorting. 2 replicates each.

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