Project description:Microarrays experiments were performed in order to assess which genes are differentially expressed between the embryonic chicken sinus horns at HH14. Three, technical, dye swapped comparisons between Left and Right sinus horns. In total six arrays were used in this study
Project description:Microarrays experiments were performed in order to assess which genes are differentially expressed between the embryonic chicken sinus horns at HH14.
Project description:We report the transcriptomes of 10 different chicken (Gallus gallus) cell/tissue types. The goal of this project was to determine similarities and differences between different cell/tissue types, with respect to protein coding genes, lncRNA, isoform counts, and differential gene expression. We provide raw data and bigWig files for UCSC visualization. The findings described here will be useful towards a complete annotation of chicken tissue and cellular transcriptomes.
Project description:HH stage 21 chicken dorsal mesentery is laser microdissected into four morphological compartments, and subject to global expression analyses on Affymetrix Chicken 3' microarray Goal is to identify genes expressed in each of left epitelium, left mesenchyme, right mesenchyme, right epithelium; genes on the left may be direct or indirect transcriptional targets of Pitx2.
Project description:To examine global gene expression profile of chicken early paraxial mesoderm differentiation, we microdissected stage 12HH chicken PSM regions into 20 pieces (10 pieces both left-right PSM), including the tail bud, the PSM and somites. We create microarray series using these fragments.
Project description:Next Generation RNA Sequencing was carried out on human paired left and right atrial appendages from patients with and without Atrial Fibrillation. EdgeR software was used to show a total of 247 genes were found to have significant differential expression between left and right atria.
Project description:C3H/HeH females were mated with 101/H males<br><br>Mating was assessed by the presence of vaginal plugs the next morning. Identification of plug was designated day 0.5.<br><br> <br><br>At 8.5 days of development the dam was culled by cervical dislocation, the uterine horns disscted and placed into PBS. The uterus was dissected to release decuduae and these opened according to standard protocols. Morphologically normal looking embryos of between 4 and 6 somites were collected. The left and right lateral plates were dissected away with watchmakers forceps and pools of 4 left and 4 right lateral plates were snap frozen in LN2.
