Project description:S. enterica sv Kentucky 3795 and S. enterica sv Enteritidis NalR were grown to mid-log phase in TSB, then subjected to three different acidic conditions generated by two different acids: HCl to pH4.5, HCl to pH5.5 and CH3COOH to pH5.5. After 10min, total RNA was harvested und compared to total RNA harvested from identical control cultures grown in TSB without the pH alteration. At least three biological replicates were performed for each strain and acidic condition. Total RNAs were harvested, and labeled by the conventional protocol of Brown et al, then hybridized onto a Salmonella-specific PCR product array that covered over 97% of the Enteritidis genome.
Project description:Purpose: Searching for sRNAs in Salmonella pullorum by RNA sequencing and exploring their functions.Methods: High-throughput sequencing of RNA extracted from Salmonella pullorum under normal growth conditions to detect newly discovered sRNAs, followed by experiments to verify their functions.Results: The proportion of Clean Reads of this sequencing was >65%, and the base Q30s were all above 85%, indicating that the sequencing quality is good and can be used for subsequent analysis. The sRNAscanner software predicted that 148 new sRNAs might exist on the reference genome of Salmonella fowl dysentery, and the reads obtained from sequencing were compared to the genome, and it was found that 110 out of the 148 newly predicted sRNAs could be detected.Conclusions: sRNAs are widely found in bacteria and are involved in many physiological processes. In this study, we detected new sRNAs in Salmonella pullorum by RNA-seq, which lays the foundation for the subsequent investigation of the regulatory functions of sRNAs in bacteria.
