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AMPK related of circRNA expression in C57 male mice myocardium

ABSTRACT: AMPK related of circRNA expression in C57 male mice myocardium

PROVIDER: PRJNA1167178 | ENA |

REPOSITORIES: ENA

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Project description:To identify adenosine monophosphate-activated protein kinase (AMPK)-related circular RNA (circRNA) that was differentially expressed in the mouse myocardial ischaemia-reperfusion injury (MIRI) model, and map the AMPK-related circRNA network to provide novel insights for the prevention and treatment of MIRI.

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Cardiac Sex Differences in the Transcriptome of C57 Male and Female Adult Hearts

Project description:Purpose: The goals of this study are to compare adult male and female C57 mouse hearts transcriptome profiling (RNA-seq) to conclude cardiac sex differences at the mRNA level. Methods: mRNA profiles of adult male and female C57 mouse hearts were generated by deep sequencing, n=4 for each sex, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 50 million sequence reads per sample to the mouse genome (build mm10) and identified 16,160 transcripts in the mouse hearts.

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