Project description:We report the application of dual RNA-sequencing technology for high-throughput profiling of histone modifications in HaCat cells and Trichophyton mentagrophytes complex.For co-culture assays, a ratio of 2.5×105 cells/mL of keratinocytes to 2.5×105 conidia/mL of T. mentagrophytes, T. interdigitale, and T. tonsurans solution were used (MOI=1). The experiment was carried out for 24 h in a humidified incubator maintained at 37 ºC . We used dual RNA-seq to study the different host immune responses against the T. mentagrophytes complex and we the transcriptional profiles of differentially expressed genes in dermatophytes.

2022-08-19 | GSE199307 | GEO

Transcriptome Sequencing and Gene Expression Analysis of Trichophyton mentagrophytes under Different Culture Conditions

Project description:Our study provides considerable gene expression information of Trichophyton mentagrophytes at the transcriptional level, which will help accelerate the research on the antifungal medicine development. Additionally, we have demonstrated the feasibility of using the Illumina sequencing based DGE system for gene expression profiling, and have shed new light on functional studies of the genes involved in antifungal mechanisms of berberine hydrochloride and clotrimazole.

2019-03-20 | GSE80604 | GEO

Trichophyton mentagrophytes complex

Project description:Genomic data of Trichophyton mentagrophytes complex strains

| PRJNA910985 | ENA

Trichophyton mentagrophytes strain:TIMM 2789

Project description:Trichophyton mentagrophytes TIMM 2789 genome project

| PRJDB6558 | ENA

Trichophyton mentagrophytes strain:28185

Project description:Trichophyton mentagrophytes strain:28185 Transcriptome or Gene expression

| PRJNA356953 | ENA