Project description:At present, only several species of genus Acentrogobius were described and information of mitochondrial DNA were also just focused on two species. Here, the complete mitochondrial genome of Acentrogobius caninus was completely sequenced by high throughput sequencing method. The complete mitochondrial genome was 16,614 bp in length, consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, 1 large non-coding control region, and 1 light-strand replication origin. Overall base composition values for the mitochondrial genome were 28.24, 29.20, 16.77, and 25.79% for A, C, G, and T, respectively. The gene arrangement is identical to those in typical fishes. Phylogenetic tree based on 13 protein-coding genes shows that three species of genus Acentrogobiu did not cluster into one clade.
Project description:A seven-year-old immunocompetent dog presenting with lymphadenopathy, mesenteric masses and splenic nodules was diagnosed with Phialosimplex caninus infection. Cytology of a mesenteric mass aspirate demonstrated few intact cells but numerous variably sized fungal cells and rare hyphal fragments. The identity of the cultured fungus was confirmed by DNA sequencing. Itraconazole therapy improved clinical signs, but the fungus was reisolated at follow-up. P. caninus systemic infection should be suspected in dogs presenting with lymphadenopathy and splenomegaly.
Project description:The DNA isolated from 44 either frozen or FFPE Neuroendocrine Neoplasm (NEN) was analysed by NGS, to identify genes more likely to be subject to sequence variations among 523 cancer-related ones.
Project description:Plasma DNA from 558 malignancies, 263 benign and borderline tumors and 367 healthy control samples were collected and subjected to random short-gun whole genome sequencing.
Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.