Project description:Ten populations were evolved for 6,000 generations. Five had strong selection for sporulation, imposed partially by their cultivation in sporulation-inducing medium, while the other five populations had relaxed selection for sporulation, by cultivating them in sporulation-repressing medium. Batch cultures were diluted 1:100 daily for approximately 892 days. In the five populations with relaxed selection for sporulation, sporulation ability was eventually lost. Keywords: comparative genome hybridization and transcriptome divergence
Project description:Ten populations were evolved for 6,000 generations. Five had strong selection for sporulation, imposed partially by their cultivation in sporulation-inducing medium, while the other five populations had relaxed selection for sporulation, by cultivating them in sporulation-repressing medium. Batch cultures were diluted 1:100 daily for approximately 892 days. In the five populations with relaxed selection for sporulation, sporulation ability was eventually lost. Keywords: comparative genome hybridization and transcriptome divergence Genomic DNA from all ten populations, and ancestors, was isolated, labeled and hybridized to microarrays. Only one hybridization was performed for each experimental population, with evolved and ancestral DNA hybridized to the slide. RNA hybridizations were done by chosing one strain that was evolved in sporulation-repressing medium and one strain that was evolved in sporulation-inducing medium. Two biological replicates were performed, as well as a dye swap for each biological replicate. Evolved and ancestral strains were grown in identical medium and RNA was isolated at the onset of stationary phase.
Project description:In general, yeast strains with myo1 knocked out cannot perform cytokinesis, but some evolve new ways of accomplishing the task. This is an examination of aneuploidies and CNVs in a selection of evolved mutants that can successfully perform cytokinesis. The purpose is to attempt a molecular characterization of the evolved responses.
Project description:In general, yeast strains with myo1 knocked out cannot perform cytokinesis, but some evolve new ways of accomplishing the task. This is an examination of differential gene expression patterns in a selection of evolved mutants that can successfully perform cytokinesis. The purpose is to attempt a molecular characterization of the evolved responses.
Project description:Endospore (hereafter called spore) is a dormant, tough, and non-reproductive structure produced in a lack of nutrients by certain species of bacteria from the Firmicute phylum. Sporulation is tightly linked to cell cycle and involving by temporal and spatial regulation of hundreds genes. It has been suggested more than 500 genes are contributed to sporulation in B. subtilis, and there are certainly more. In our study, we identified four new sporulation genes and their mutants exhibited sporulation abnormal. RNA-seq of mutants compared to wild type during sporulation will reveal the regulation of sporulation related genes in the four mutants, and therefore shed the light to the functional role of the four new sporulation genes.
Project description:To characterize the defense mechanisms P. aeruginosa has evolved in response to its most toxic phenazine, pyocyanin, we performed a genome-wide transposon sequencing (TnSeq) screen in which the mutant library was exposed to PYO under carbon starvation in order to maximize PYO toxicity, and genes for which transposon mutants were significantly enriched or depleted were identified.
