Project description:PREMISE OF THE STUDY:Microsatellite markers were developed for Vatica mangachapoi (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China. METHODS AND RESULTS:Twenty microsatellite markers, including 12 polymorphic markers, were identified from V. mangachapoi using high-throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species V. guangxiensis. CONCLUSIONS:These markers may be used to investigate the genetic diversity and gene flow of V. mangachapoi and V. guangxiensis.
Project description:Vatica najibiana Ummul-Nazrah (Dipterocarpaceae), from the Relai Forest Reserve, Gua Musang, Kelantan and Gua Tanggang, Merapoh, Pahang, is described and illustrated. This species is Endangered and known from small populations restricted to two isolated karst limestone hills. The type locality, Relai Forest Reserve limestone, is currently under threat from encroaching oil palm plantations and ongoing logging, which, if it continues, will threaten the Kelantan population with extinction. The morphology of V. najibiana and the similar V. odorata subsp. odorata and V. harmandiana is compared.
| S-EPMC5958174 | biostudies-literature
Project description:Genotyping by sequencing of Vatica guangxiensis
