Project description:Purpose: We aim to reveal maize transcriptomic changes with water and salinity treatment. Methods: RNA-seq were used to reveal transcriptome of maize biological replicates with water and salinity treatment. Results: Differentially expressed transcripts were identified by the comparison of biological replicates with water and salinity treatment. Conclusions: We identified differentially expressed genes in respone to salinity treatment in maize.
Project description:Transcriptome sequencing (RNA-seq) was used to profile genome-wide transcript abundance in the primary root growth zone (PRGZ) of maize seedlings grown in different water deficit treatments: well-watered (-0.02 MPa), mild water deficit stress (-0.3 MPa), or severe water deficit stress (-1.6 MPa). For each water deficit treatment, the PRGZ transcriptome was profiled at 26 hours after initiation of the water deficit treatment. By comparing the abundance of each transcript under mild or severe water deficit stress relative to its abundance under well-watered conditions, we identified transcripts that are differentially regulated in the PRGZ in response to the two levels of water deficit stress.
Project description:The study was conducted to identify differentially expressed polyethylene glycol (PEG) induced water stress responsive genes in E. grandis. Forty day old rooted cutting of E. grandis was subjected to -0.225 MPa PEG treatment and total RNA was isolated from leaves of water treated control and PEG treated samples after three hours of treatment. The differential expression of water stress responsive genes was analyzed using microarray technique.
2016-01-01 | GSE73106 | GEO
Project description:Water Treatment Unit Breadboard
Project description:Two Clade E Growth Regulating PP2Cs EGR1 and EGR2 (EGR1, At3g05640; EGR2, At5g27930) are strongly up regulated by low water but much less affected by ABA. EGR mutants maintained higher seedling root elongation and dry weight at low water potential and higher levels of stress protective metabolite proline. Phosphoproteomics of egr1egr2 double mutant showed an increased phosphopeptide abundance of several cytoskeleton and plasma membrane-associated proteins, and consistent to this, egr mutants had more extensive microtubules recovery during low water potential acclimation. Microarray experiments were used to identify genes differentially expressed in egr1-1egr1-2 (SALK_011589/salk_048861) versus wild type under both unstress control conditions and after low water potential (ie: drought, water deficit). A relatively long term (96 h) low water potential treatment was used as phenotypes of egr1-1egr2-1 were most apparent after this longer term low water potential treatment.
Project description:The Clade A PP2C Highly ABA-Induced1 (HAI1, At5g59220) is strongly up-regulated by low water potential in an ABA-dependent manner. Using knockout mutants of hai1, we found that HAI1 functions as a negative regulator of low water potential-induced proline and osmoregulatory solute accumulation. We also found a relatively weak and limited interaction of HAI1 with the RCAR/PYL family of ABA receptors. This, plus its induced expression, suggest that HAI1 remains active during stress and attenuates specific aspects of drought response. Microarray experiments were used to identify genes differentially expressed in hai1-2 (SALK_108282) versus wild type under both unstress control conditions and after low water potential (i.e., drought, water deficit). A relatively long term (96 h) low water potential treatment was used as phenotypes of hai1-2 were most apparent after this longer term low water potential treatment.
2012-02-03 | GSE35258 | GEO
Project description:Synthetic Eutrophic Water Treatment Study