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Discovery and analysis of NFE2L1's non-transcriptional activity by transcriptome sequencing in hepatocellular carcinoma HepG2 cells

ABSTRACT: Discovery and analysis of NFE2L1's non-transcriptional activity by transcriptome sequencing in hepatocellular carcinoma HepG2 cells

PROVIDER: PRJNA1177427 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31104700_1.fastq.gz	Fastqsanger.gz
	SRR31104700_2.fastq.gz	Fastqsanger.gz
	SRR31104701_1.fastq.gz	Fastqsanger.gz
	SRR31104701_2.fastq.gz	Fastqsanger.gz
	SRR31104702_1.fastq.gz	Fastqsanger.gz

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Discovery and analysis of NFE2L1's non-transcriptional activity by transcriptome sequencing in hepatocellular carcinoma HepG2 cells

Project description:Antitumor transcription activator NFE2L1 regulates redox homeostasis, protein turnover, and material metabolism, playing a crucial role in embryonic development and the specialization of tissue and organ functions. Deficiency of the NFE2L1 gene in different regions yields distinct phenotypes, suggesting that NFE2L1 may possess functions independent of its transcription factor activity. In this study, we originally discovered the non-transcription factor activity of NFE2L1 by constructing a truncated protein, NFE2L1ΔC, which lacks the 152 amino acids at the C-terminus and loses its transcription factor activity. By studying the function of the truncated NFE2L1 protein, we revealed that the suppressor effect of NFE2L1 on tumor cells does not require its transcription factor activity and nuclear localization. Then, transcriptome sequencing was performed to decipher the function of NFE2L1, and the results revealed that NFE2L1 regulated redox homeostasis, proteasome function, and antiviral and proinflammatory responses primarily through its transcriptional activation function in the nucleus. In contrast, NFE2L1’s regulation of developmental related signals, ribosome function, and canceration were closely associated with its non-transcription factor activity in the cytoplasm. In this study, the non-transcription factor activity of NFE2L1 was discovered and described for the first time, laying a foundation for both basic and applied research on NFE2L1.

2024-10-25 | GSE280238 | GEO

Expression data from hepatocellular carcinoma cell lines

Project description:In this study, we demonstrated that mitochondrial respiratory defect enhanced NFE2L1 transcription via reactive oxygen species (ROS)-mediated STAT3 activation and the up-expressed NFE2L1 increased hepatoma cell invasiveness by inducing syntaxin 12 (STX12) expression. Bioinformatic analysis of The Cancer Genome Atlas Liver Hepatocellular carcinoma (TCGA-LIHC) open database showed that NFE2L1 expression is strongly positively correlated with STX12 expression and, furthermore, epithelial-mesenchymal transition (EMT)-related core genes were significantly upregulated in the tumors expressing both NFE2L1 and STX12. The effect of NFE2L1/STX12 axis on lung metastasis of hepatoma cell was proved in nude mice model. Collectively, our results indicate that NFE2L1 was a key mitochondrial retrograde signaling-mediated primary gene product to enhance hepatoma cell invasiveness via STX12 expression

2020-09-09 | GSE137054 | GEO

DDX20 knockdown effect on hepatocellular carcinoma cells

Project description:Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells. One condition experiment, HepG2 vs. HepG2-DDX20 knockdown cells

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Expression analysis of HepG2, HepG2-slug and HepG2-slug on Matrigel

Project description:Investigation of whole genome gene expression level changes in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Whole genome gene expression level changes have been compared in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Roche NimbleGen micro-array analysis was employed to assess global genome expression in HepG2 in regular culture, HepG2-slug in regular culture and HepG2-slug on Matrigel. The results demonstrated that the up-regulated genes and the down-regulated genes increased significantly when HepG2-slug cells with VM forming ablity were cultured on Matrigel and formed VM.

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Impact of NFE2L1 knockout on gene expression

Project description:These files represent DMSO and bortezomib (200 nM, 4 h) treated Control (B2) and NFE2L1 KO1 (B4) and NFE2L1 KO2 (B6) A549 cells.

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RNA Sequencing of human liver hepatocellular carcinoma cell line HepG2 transcriptomes

Project description:RNA Sequencing of human liver hepatocellular carcinoma cell line HepG2 transcriptomes

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Genes regulated by GSK343 in HepG2 and PLC5 hepatocellular carcinoma cells

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Genes directly regulated by NF-κB in human hepatocellular carcinoma HepG2

Project description:Genes directly regulated by NF-κB in human hepatocellular carcinoma HepG2

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