Saccharomyces cerevisiae
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ABSTRACT:
PROVIDER: PRJNA118051 | ENA |
REPOSITORIES: ENA
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Project description:Resistance of Saccharomyces cerevisiae to high furfural concentration is based on NADPH-dependent reduction by at least two oxireductases. Biofuels derived from lignocellulosic biomass hold promises for a sustainable fuel economy, but several problems hamper their economical feasibility. One important problem is the presence of toxic compounds in processed lignocellulosic hydrolysates with furfural as a key toxin. While Saccharomyces cerevisiae has some intrinsic ability to reduce furfural to the less toxic furfuryl alcohol, higher resistance is necessary for process conditions. By comparing an evolved, furfural resistant strain and its parent in micro-aerobic, glucose-limited chemostats at increasing furfural challenge, we elucidate key mechanism and the molecular basis of both natural and high-level furfural resistance. At lower furfural concentrations, NADH-dependent oxireductases are the main defence mechanism. At concentrations above 15 mM, however, [1-13C]-flux and global array-based transcript analysis demonstrated that the NADPH-generating flux through pentose-phosphate pathway increases and that NADPH-dependent oxireductases became the major resistance mechanism. The transcript analysis further revealed that iron transmembrane transport is up-regulated in response to furfural. While these responses occur in both strains, high-level resistance in the evolved strain was based on strong induction of ADH7, the uncharacterised ORF YKL071W and 4 further, likely NADPH-dependent oxireductases. By overexpressing the ADH7 gene and the ORF YKL071W, we inverse engineered significantly increased furfural resistance in the parent strain, thereby demonstrating these two enzymes to be key elements of the resistance phenotype.
2009-09-29 | GSE18314 | GEO
Project description:Resistance of Saccharomyces cerevisiae to high furfural concentration is based on NADPH-dependent reduction by at least two oxireductases. Biofuels derived from lignocellulosic biomass hold promises for a sustainable fuel economy, but several problems hamper their economical feasibility. One important problem is the presence of toxic compounds in processed lignocellulosic hydrolysates with furfural as a key toxin. While Saccharomyces cerevisiae has some intrinsic ability to reduce furfural to the less toxic furfuryl alcohol, higher resistance is necessary for process conditions. By comparing an evolved, furfural resistant strain and its parent in micro-aerobic, glucose-limited chemostats at increasing furfural challenge, we elucidate key mechanism and the molecular basis of both natural and high-level furfural resistance. At lower furfural concentrations, NADH-dependent oxireductases are the main defence mechanism. At concentrations above 15 mM, however, [1-13C]-flux and global array-based transcript analysis demonstrated that the NADPH-generating flux through pentose-phosphate pathway increases and that NADPH-dependent oxireductases became the major resistance mechanism. The transcript analysis further revealed that iron transmembrane transport is up-regulated in response to furfural. While these responses occur in both strains, high-level resistance in the evolved strain was based on strong induction of ADH7, the uncharacterised ORF YKL071W and 4 further, likely NADPH-dependent oxireductases. By overexpressing the ADH7 gene and the ORF YKL071W, we inverse engineered significantly increased furfural resistance in the parent strain, thereby demonstrating these two enzymes to be key elements of the resistance phenotype. Experiment Overall Design: RNA levels were measured in glucose limited, micro-aerobic chemostat cultures with different concentrations of the growth inhibitor furfural. Two strains were compared: TMB3400-FT30-3 is a strain that has been evolutionary adapted to withstand high furfural concentrations. TMB3400 is its less resistant parent. Number of biological replicates: 2-3.
2009-10-03 | E-GEOD-18314 | biostudies-arrayexpress
Project description:HMF and furfural were pulse added to xylose-utilizing Saccharomyces cerevisiae during either the glucose consumption phase or the xylose consumption phase. Transcriptome samples were collected before and one hour after pulsing of inhibitors.
2013-11-25 | GSE50182 | GEO
Project description:Saccharomyces cerevisiae stress responses to HMF and furfural
| PRJNA177843 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE34808: A transcriptomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation [2009] GSE34809: A transcriptomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation [2010] Refer to individual Series
2012-03-31 | E-GEOD-34810 | biostudies-arrayexpress
Project description:We analyzed genome-wide transcriptional changes in the S. cerevisiae strain BY4742 upon exposure to NP to identify differentially expressed genes, biological processes, metabolic pathways, and cellular compartments affected by this compound. For these analyses, we focused on two NP exposure scenarios: (1) exposure to a low inhibitory concentration (resulting in <15% reduction in cell number), and (2) exposure to a high inhibitory concentration (resulting in >65% reduction in cell number).
2020-03-01 | GSE96651 | GEO
Project description:HMF and furfural were pulse added to xylose-utilizing Saccharomyces cerevisiae during either the glucose consumption phase or the xylose consumption phase. Transcriptome samples were collected before and one hour after pulsing of inhibitors. Three biological replicates from each conditions analyzed.
2013-11-25 | E-GEOD-50182 | biostudies-arrayexpress
Project description:Furfural is a key inhibitor in S. cerevisiae fermentation causing serious economic loss. To understand the toxic mechanisms of furfural-induced genomic instability and phenotypic evolution, we mapped chromosomal alterations in 21 furfural-treated yeast strains by whole genome SNP microarrays at a resolution about 1kb.
2017-10-13 | GSE104879 | GEO
Project description:Saccharomyces cerevisiae stress responses to HMF and furfural (xylose and glucose) Oct
| PRJNA216986 | ENA
Project description:A transcriptomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation [2009]
| PRJNA156289 | ENA