Project description:First High-Density Linkage Map and QTL Fine Mapping for Growth and Alkalinity Tolerance of Spotted Sea Bass (Lateolabrax maculatus)
Project description:We have further tested and validated a reproduction-enriched microarray designed specifically for European sea bass providing a reliable tool to study gene expression patterns in this fish species. Results obtained by using this platform helped to increase our knowledge in the gene cascade of sex differentiation and give evidence of the complexity of the molecular processes. We described the gene expression profile of genes and pathways required to differentiate bipotential tissue to either an ovary or testis providing a deep transcriptomic analysis in the developing gonads. Resulting data may help to improve sex control in fish culture particularly in the European sea bass
Project description:A sea bass oligo microarray platform was used to profile gene expression in whole heads of 38 days-old sea bass affected by prognathism, a skeletal malformation that strongly affects sea bass production. Two different conditions: i) prognathous individuals, and ii) normal individuals were analyzed. For each condition, total RNA was extracted from three (3) independent biological replicates, each consisting of pools of five (5) heads.
