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Fusarium oxysporum f. sp. lycopersici

ABSTRACT: Fusarium oxysporum f. sp. lycopersici Raw sequence reads

PROVIDER: PRJNA1182093 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR31238313_1.fastq.gz	Fastqsanger.gz
	SRR31238313_2.fastq.gz	Fastqsanger.gz
	SRR31238314_1.fastq.gz	Fastqsanger.gz
	SRR31238314_2.fastq.gz	Fastqsanger.gz
	SRR31238315_1.fastq.gz	Fastqsanger.gz

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Project description:To further understand the abnormal phenotype of the calcineurin mutant strains and identify calcineurin-mediated genes in F. oxysporum f. sp. lycopersici, we performed RNA sequencing to compare the transcriptome profiles of the wild-type (WT), Δcna1(HFW1) and Δcnb1 (HFW3) mutants. By pairwise analysis, a total of 2139 genes were differentially expressed between WT and Δcna1 mutant based on the selection criteria of four-fold change in expression (Log2 fold change > 2 or < -2, P < 0.05). Among these, 1079 genes were upregulated and 1060 genes were downregulated. Moreover, from the comparison of WT against Δcnb1 mutant, 1412 gene were being regulated, and 840 genes were upregulated while 572 genes were downregulated. To elucidate genes that were regulated by calcineurin in F. oxysporum f. sp. lycopersici, the gene sets obtained from the pairwise analyses were compared, giving an overlap of 737 genes. Among these, 414 genes were found to be downregulated and 323 were upregulated, indicating that these genes possibly involved in the calcineurin pathway. GO functional analysis showed these transcripts were mainly involved in the oxidation-reduction process, single-organism metabolic process, transporter activity, cofactor binding, and other metabolic and biological processes.

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Project description:Fusarium oxysporum f. sp. lycopersici 4287 Raw sequence reads

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Project description:Fusarium oxysporum f. sp. lycopersici 4287 Raw sequence reads

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Comparsion of histone modifications of core and accessory chromosomes of Fusarium oxysporum

Project description:To study whether there are differences in chromatin-mediated regulation between and within chromosomes in Fusarium oxysporum f. sp. lycopersici 4287 (FGSC9935), we determined the distribution of histone marks associated with euchromatin (H3K4me2) and facultative heterochromatin (H3K27me3) in vitro. We then determined whether these differences correlate with differences in dispensability and sequence divergence and gene expression.

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