ABSTRACT: POTENTIAL OCCUPATIONAL EXPOSURE TO OPPORTUNISTIC BACTERIAL PATHOGENS IN BIOAEROSOLS EMITTED AT MUNICIPAL WASTEWATER TREATMENT PLANTS, SOUTH AFRICA
Project description:Municipal wastewater effluent can impact its receiving environment. In the St. Lawrence River, male fish living downstream from Montreal exhibit increased hepatic vitellogenin, intersex, delayed spermatogenesis and altered immune function. Few studies have examined genome-wide effects associated with municipal effluent exposure in fish to decipher the mechanisms of toxicity. The present objective was to identify hepatic cellular signaling pathways in fathead minnows following exposure to municipal wastewater effluent. Immature minnows were exposed for 21 days to either 0% (Control) or 20% municipal effluent, the highest concentration in the St. Lawrence River. Hepatic RNA was extracted and used to hybridize a fathead minnow oligonucleotide microarray containing approximately 15K gene sequences.
2014-08-31 | GSE52693 | GEO
Project description:Bioaerosols from wastewater treatment plants
Project description:Municipal wastewater effluent can impact its receiving environment. In the St. Lawrence River, male fish living downstream from Montreal exhibit increased hepatic vitellogenin, intersex, delayed spermatogenesis and altered immune function. Few studies have examined genome-wide effects associated with municipal effluent exposure in fish to decipher the mechanisms of toxicity. The present objective was to identify hepatic cellular signaling pathways in fathead minnows following exposure to municipal wastewater effluent. Immature minnows were exposed for 21 days to either 0% (Control) or 20% municipal effluent, the highest concentration in the St. Lawrence River. Hepatic RNA was extracted and used to hybridize a fathead minnow oligonucleotide microarray containing approximately 15K gene sequences. Sixteen samples were examined, 8 control samples and 8 exposed samples.
Project description:In this study, we exposed Caenorhabditis elegans wild types N2 to water collected from six sources in the Dutch village Sneek. The sources were: wastewater from a hospital, a community (80 households), a nursing home, influent into the local municipal wastewater treatment plant, effluent of the wastewater treatment plant, and surface water samples. The goal of the experiment was to determine if C. elegans can be used to identify pollutants in the water by transcriptional profiling. Age synchronized worms at developmental L4 larval stage were exposed to treatment for 24 hours. After flash freezing the samples, RNA was isolated, labeled and hybridized on oligo microarray (Agilent) slides.
Project description:Printer emitted particles (PEPs)-induced cardiopulmonary adverse effects and health risks and the underlying mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in rat blood to identify non-invasive blood based biomarkers for medical and occupational surveillance. The rats were exposed to printer emitted particles PEPs (exposed) and high efficiency particulate air (HEPA) filtered air (control). Animals were sacrificed on 1, 5, 9, and 21 days for lung tissue collection.
Project description:Printer emitted particles (PEPs)-induced cardiopulmonary adverse effects and health risks and the underlying mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in rat blood to identify non-invasive blood based biomarkers for medical and occupational surveillance. The rats were exposed to printer emitted particles PEPs (exposed) and high efficiency particulate air (HEPA) filtered air (control). Animals were sacrificed on 1, 5, 9, and 21 days for lung tissue collection.
Project description:Printer emitted particles (PEPs)-induced cardiopulmonary adverse effects and health risks and the underlying mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in rat blood to identify non-invasive blood based biomarkers for medical and occupational surveillance. The rats were exposed to printer emitted particles PEPs (exposed) and high efficiency particulate air (HEPA) filtered air (control). Animals were sacrificed on 1, 5, 9, 13, 17 and 21 days for blood collection.
Project description:Printer emitted particles (PEPs)-induced cardiopulmonary adverse effects and health risks and the underlying mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in rat blood to identify non-invasive blood based biomarkers for medical and occupational surveillance. The rats were exposed to printer emitted particles PEPs (exposed) and high efficiency particulate air (HEPA) filtered air (control). Animals were sacrificed on 1, 5, 9, 13, 17 and 21 days for blood collection.
