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Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

ABSTRACT: Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

PROVIDER: PRJNA1183369 | ENA |

REPOSITORIES: ENA

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Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Screening of differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection. We independently transfected SW1990 and Panc-1 cells with Gli1 cDNA for mRNA and miRNA microarray analysis. The results unveiled significant differential expression patterns, with numerous miRNAs and mRNAs showing changes post-Gli1 transfection. Remarkably, miR-301a exhibited high expression in both SW1990 and Panc-1 cells transfected with Gli1 cDNA

2025-01-29 | GSE281316 | GEO

Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Screening of differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection. We independently transfected SW1990 and Panc-1 cells with Gli1 cDNA for mRNA and miRNA microarray analysis. The results unveiled significant differential expression patterns, with numerous miRNAs and mRNAs showing changes post-Gli1 transfection. Remarkably, miR-301a exhibited high expression in both SW1990 and Panc-1 cells transfected with Gli1 cDNA.

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Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

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Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

Project description:Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array GLI1 direct target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 3 hours after treatment of beta-estradiol (E2) at doses of 10nM.

2013-07-18 | E-GEOD-28901 | biostudies-arrayexpress

Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

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Project description:Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array

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Identification of ZIC2 target genes in human pancreatic cancer cell lines PANC-1 and KP-4

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GLI1 knock-down in Panc1 cells

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The differences between CD166+ pancreatic cancer cells and CD166- cells

Project description:Microarray analyses using CD166+ and CD166- pancreatic cancer cell lines from both Panc-1 and SW1990 cells. We carried out microarray analyses using the CD166+ and CD166- separated cells from both Panc-1 and SW1990. The quality of the RNA samples was evaluated using a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). We used a Human HT-12v4 Expression BeadChip (Illumina, San Diego, CA) for these analyses. The data were analyzed using BeadStudio software version 3.2.3 (Illumina).

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