Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA118419 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster
| PRJNA317422 | ENA
Project description:Selenophosphate, which is the active donor of selenium in selenocysteine (Sec) biosynthesis is synthesized from selenite and ATP by an enzyme designated as selenophosphate synthetase (SPS).There are two isoforms of SPS in higher eukaryotes,SPS1 and SPS2. Initially, both SPS1 and SPS2 were thought to be involved in selenophosphate synthesis. However, it was subsequently shown that only SPS2 catalyzes selenophosphate synthesis. Although SPS1 is an essential gene in Drosophila, its function has not been determined. To identify transcriptional profile of SPS1 knockdown cells, which could offer valuable clues to identify molecular mechanism of SPS1, we introduced RNAi using its cognate double-stranded RNA into Drosophila SL2 cells, and then microarray analysis was carried out. Experiment Overall Design: In order to find primary or secondary target gene which is regulated by SPS1,Drosophila SL2 cells were harvested at day1, day3 and day5 after SPS1 knockown for RNA extraction and hybridization on Affymetrix microarrays.
2009-08-22 | E-GEOD-17685 | biostudies-arrayexpress
Project description:Selenophosphate, which is the active donor of selenium in selenocysteine (Sec) biosynthesis is synthesized from selenite and ATP by an enzyme designated as selenophosphate synthetase (SPS).There are two isoforms of SPS in higher eukaryotes,SPS1 and SPS2. Initially, both SPS1 and SPS2 were thought to be involved in selenophosphate synthesis. However, it was subsequently shown that only SPS2 catalyzes selenophosphate synthesis. Although SPS1 is an essential gene in Drosophila, its function has not been determined. To identify transcriptional profile of SPS1 knockdown cells, which could offer valuable clues to identify molecular mechanism of SPS1, we introduced RNAi using its cognate double-stranded RNA into Drosophila SL2 cells, and then microarray analysis was carried out.
2009-08-18 | GSE17685 | GEO
Project description:In mammalian cells, AU-rich elements (AREs) are well known regulatory sequences located in the 3' untranslated region (UTR) of many short-lived mRNAs that suppress gene expression at the posttranscriptional level. Tis11, a zinc finger RNA-binding protein homologous to mammalian tristetraprolin, targets ARE-containing reporter mRNAs for rapid degradation in SL2 cells. To identify Drosophila mRNA targets of Tis11, we performed genome-wide expression profiling after dsRNA-mediated depletion of endogenous Tis11 in SL2 cells. Furthermore, we studied the involvement of Tis11 in regulating the Drosophila immune response by profiling mRNA expression after LPS treatment, in the presence or absence of Tis11.
2011-03-25 | GSE28147 | GEO
Project description:In mammalian cells, AU-rich elements (AREs) are well known regulatory sequences located in the 3' untranslated region (UTR) of many short-lived mRNAs that suppress gene expression at the posttranscriptional level. Tis11, a zinc finger RNA-binding protein homologous to mammalian tristetraprolin, targets ARE-containing reporter mRNAs for rapid degradation in SL2 cells. To identify Drosophila mRNA targets of Tis11, we performed genome-wide expression profiling after dsRNA-mediated depletion of endogenous Tis11 in SL2 cells. Furthermore, we studied the involvement of Tis11 in regulating the Drosophila immune response by profiling mRNA expression after LPS treatment, in the presence or absence of Tis11. SL2 cells were treated with either dsRNA against Tis11 or dsRNA against GFP (control). After 4 days of treatment with 12.5 microgram dsRNA / ml medium, total RNA was extracted and hybridized to Drosophila Genome 2.0 Arrays (Affymetrix, Cat. No. 900531). Where indicated, cells were treated with lipopolysaccharide (LPS, Sigma, O55:B5) at a concentration of 10 microgram / ml for 4 hours before lysis. All experiments were performed in 3 biological replicates.
2011-03-24 | E-GEOD-28147 | biostudies-arrayexpress
Project description:Profiling of changes in steady state RNA levels upon RNAi-mediated knockdown of the nucleosome remodeling ATPase ISWI in Drosophila SL2 cells. Quantitation of the corresponding proteomes has been performed to describe transcriptome-proteome relations. The analysis indicates a limited correlation of the two expression steps. Keywords: RNAi-mediated knockdown
2008-09-05 | GSE11164 | GEO
Project description:Gene expression profile of frazzled (fra) mutant Drosophila embryos
| PRJNA203645 | ENA
Project description:Profiling of changes in steady state RNA levels upon RNAi-mediated knockdown of the nucleosome remodeling ATPase ISWI in Drosophila SL2 cells. Quantitation of the corresponding proteomes has been performed to describe transcriptome-proteome relations. The analysis indicates a limited correlation of the two expression steps. Experiment Overall Design: Drosophila SL2 cells were incubated 7 days after treatment with 10 ug of dsRNA directed against GST or ISWI, respectively. 3 biological replicates per experimental conditions have been collected.
2008-10-25 | E-GEOD-11164 | biostudies-arrayexpress
Project description:Drosophila melanogaster nxf2 knockdown
| PRJNA606690 | ENA