Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of CTBP dehydrogenase inhibitors MTOB and 4-Cl-HIPP in J774.1 cells

ABSTRACT: The effect of CTBP dehydrogenase inhibitors MTOB and 4-Cl-HIPP in J774.1 cells

PROVIDER: PRJNA1185200 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31316000_1.fastq.gz	Fastqsanger.gz
	SRR31316000_2.fastq.gz	Fastqsanger.gz
	SRR31316001_1.fastq.gz	Fastqsanger.gz
	SRR31316001_2.fastq.gz	Fastqsanger.gz
	SRR31316002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

The effect of CTBP dehydrogenase inhibitors MTOB and 4-Cl-HIPP in J774.1 cells

Project description:C-terminal binding proteins (CTBPs) are conserved transcriptional repressors involved in cancer and inflammation. Uniquely amongst transcriptional coregulators, CTBPs possess a functional dehydrogenase domain. Since multiple malignancies display elevated CTBP levels, CTBP inhibitors targeting this dehydrogenase domain have been developed. While the importance of CTBPs dehydrogenase function for transcriptional regulation remains unclear, multiple studies have relied CTBP inhibitors such as MTOB and 4-Cl-HIPP. In vitro studies have confirmed binding of these compounds to CTBP’s active site, however evidence for specificity is currently lacking. To address this, we treated wildtype or Ctbp1, 2 double knockout J774.1 cells with MTOB or 4-Cl-HIPP and performed RNAseq. We observed that both inhibitors elicit distinct transcriptional changes indicating non-overlapping modes of action. Moreover, the majority of changes induced by either inhibitor are observed in knockout cells indicative of off-target effects. We hypothesize that those CTBP dehydrogenase inhibitors might lack specificity to CTBPs.

2025-02-25 | GSE281668 | GEO

Effect of kncoking down CtBP and TRIM28 on gene expreession in MCF-7 cells

Project description:Both TRIM28 and CtBP are important transcriptional repressors and always shown high expression in many types of cancers, in our current study, we found TRIM28 interacts with CtBP in breast cancer and normal cells. To elucidate the functions of interaction between TRIM28 and CtBP in breast cancer progression, we performed the RNAseq of knocking down TRIM28, CtBP and Ctrl(NC) genes in MCF-7 cell line.

2025-01-18 | GSE287092 | GEO

Expression data from CtBP knockdown MCF-7 cells

Project description:CtBP is a global co-repressor by serving as transcriptional factor in multiple pathways. CtBP functioned as transcriptional factor by recruiting other cofactors such as G9a, HDAC1 and PcG proteins. CtBP is found to be over enriched in several type of tumor samples. To dipict the role of CtBP in globally regulating gene expression, we applied gene microarray technology to find out what subgroups of genes are mainly affected. 6 MCF-7 cell samples, 3 with CtBP knockdown and 3 with control knock down.

2013-03-20 | E-GEOD-36529 | biostudies-arrayexpress

Metabolic effects of novel aldehyde dehydrogenase inhibitors (ALDH) inhibitors

Project description:Effect of novel aldehyde dehydrogenase inhibitors. Before an experiment medium was replaced to medium without glucose with addition of labeled glucose on C13. Next, cells were treated with ALDH inhibitors 673 or 773 for 1, 4, or 8 hr (673&773-Jan26)

2017-06-19 | ST000774 | MetabolomicsWorkbench

Expression data from CtBP knockdown MCF-7 cells

2013-03-20 | GSE36529 | GEO

Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7

Project description:CtBP is a global co-repressor by serving as transcriptional factor in multiple pathways. CtBP functioned as transcriptional factor by recruiting other cofactors such as G9a, HDAC1 and PcG proteins. CtBP is found to be over enriched in several type of tumor samples. To dipict the role of CtBP in globally regulating gene expression, we applied ChIP-seq technology to find out the binding profile of CtBP in breast cancer cell line MCF-7. Our data suggest CtBP plays a global regulatory role in DNA damage repair, tumor initiation, and EMT process. Examine CtBP binding in MCF-7 cells

2013-03-20 | E-GEOD-36546 | biostudies-arrayexpress

Project description:Drosophila melanogaster cl, clot, is expressed in 3 baseline experiment(s);

| FBgn0000318 | ExpressionAtlas

Project description:Drosophila melanogaster cl, clot, is differentially expressed in 12 experiment(s);

| FBgn0000318 | ExpressionAtlas

Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7

Project description:CtBP is a global co-repressor by serving as transcriptional factor in multiple pathways. CtBP functioned as transcriptional factor by recruiting other cofactors such as G9a, HDAC1 and PcG proteins. CtBP is found to be over enriched in several type of tumor samples. To dipict the role of CtBP in globally regulating gene expression, we applied ChIP-seq technology to find out the binding profile of CtBP in breast cancer cell line MCF-7. Our data suggest CtBP plays a global regulatory role in DNA damage repair, tumor initiation, and EMT process.

2013-03-20 | GSE36546 | GEO

CtBP

Project description:Drosophila melanogaster CtBP, C-terminal Binding Protein, is differentially expressed in 12 experiment(s);

| FBgn0020496 | ExpressionAtlas