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Genome-wide transcriptional regulation by the flagellar sigma factor FliA in Pseudomonas putida

ABSTRACT: Genome-wide transcriptional regulation by the flagellar sigma factor FliA in Pseudomonas putida

PROVIDER: PRJNA1185758 | ENA |

REPOSITORIES: ENA

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Genome-wide transcriptional regulation by the flagellar sigma factor FliA in Pseudomonas putida

Project description:The sigma factor FliA (σ28) has been described to activate the expression of several chemoreceptor-encoding genes and the late flagellar genes in Pseudomonas putida, enabling synthesis of the filament, an stator complex and completion of the flagella-associated chemotaxis machinery. The activity of FliA is repressed in the cytoplasm by the anti-sigma factor FlgM upon completion of the flagellar hook. In this study we aim to identify genome-wide targets of regulation by FliA in P. putida KT2442 (a spontaneous rifampicin-resistant mutant of the reference strain KT2440) by performing RNA-seq experiments using a fliA deletion mutant and a constitutively active strain that combines the deletion of flgM with ectopic production of FliA.

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