Effects of physical activity reduction on the gastrocnemius transcriptome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1185812 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR31341620_1.fastq.gz | Fastqsanger.gz | |||
| SRR31341620_2.fastq.gz | Fastqsanger.gz | |||
| SRR31341621_1.fastq.gz | Fastqsanger.gz | |||
| SRR31341621_2.fastq.gz | Fastqsanger.gz | |||
| SRR31341622_1.fastq.gz | Fastqsanger.gz |
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Project description:Insufficient physical activity is associated with increased relative risk of cardiometabolic disease and is an independent risk factor for mortality. Experimentally reducing physical activity rapidly induces insulin resistance, impairs glucose handling, and drives metabolic inflexibility. These adaptations manifest during the early stages of physical inactivity, even when energy balance is maintained, suggesting that inactivity-mediated metabolic reprogramming is an early event that precedes changes in body composition. To identify mechanisms that promote metabolic adaptations associated with physical inactivity, we developed a mouse model of physical activity reduction that permits the study of inactivity in animals prior to the onset of overt changes in body composition. Adult mice were randomized into three groups: an inactive control group (standard housing), an active control group (treadmill running for 6-weeks), and an activity reduction group (treadmill running for 4-weeks, followed by 2-weeks of inactivity). Transcriptional profiling of gastrocnemius muscle was performed.
2024-11-18 | GSE281849 | GEO
Project description:A regular physical training is known to contribute to preserve muscle mass and strength, maintaining structure and function of neural and vascular compartments and preventing muscle insulin resistance and inflammation. However, physical activity is progressively reduced during aging causing mobility limitations and poor quality of life. Although physical exercise for rehabilitation purposes (e.g., after fractures or cardiovascular events) or simply aiming to counteract the development of sarcopenia is frequently advised by physicians, nevertheless few data are available on the targets and the global effects on the muscle organ of adapted exercise especially if started at old age. To contribute answering this question for medical translational purposes, the proteomic profile of the gastrocnemius muscle was analyzed in 24-month-old mice undergoing adapted physical training on a treadmill for 12 weeks or kept under a sedentary lifestyle condition. Proteomic data were implemented by morphological and morphometrical ultrastructural evaluations. Data demonstrate that muscles can respond to adapted physical training started at old age, positively modulating their morphology and the proteomic profile fostering protective and saving mechanisms either involving muscle cell components and pathways (i.e., mitochondrial processes, translation pathways and protein refolding, contractile apparatus) as well as the extracellular compartment. Therefore, this study provides important insights on the targets of adapted physical training, which can be regarded as suitable benchmarks for future in vivo studies further exploring the effects of this type of physical activity by functional/metabolic approaches.
2023-11-06 | PXD045483 | Pride
Project description:* feasibility of physical activity
* increase of physical conversation
* Quality of Life (optional)
* vascular and metabolic effects
| 2095740 | ecrin-mdr-crc
Project description:Gene expression changes in Peripheral Blood Mononuclear cells (PBMC) induced by physical activity was investigated in sedentary middle-aged men (mean age 52.6 years and BMI 29.1) who undertook a 24-week physical activity programme with blood sampling in the pre-exercise period , at the end of 24-weeks prescribed physical activity , and following a two-week detraining period. AgilentTM Whole Human Genome Oligo Microarrays were utilised to examine the effects of physical activity on mRNA expression profiles of the Peripheral Blood Mononuclear cells (PBMC) at 3 time points (pre-exercise, after 24 weeks physical activity, and at 26 weeks after 2 weeks detraining. There were 12 participants in this programme.
2010-08-03 | E-GEOD-12385 | biostudies-arrayexpress
Project description:A number of chronic, age-related diseases are associated with elevated markers of inflammation such as interleukin-6 (IL-6). In this study, we investigated the hypothesis that sedentary individuals with disparate basal serum IL-6 respond differentially to a structured physical activity programme. Gene expression changes in Peripheral Blood Mononuclear cells (PBMC) induced by physical activity was investigated in sedentary, middle-aged men (mean age 52.6 years and BMI 29.1), with relatively high or low basal serum IL-6 levels (mean of 2.13 and 0.59pg/ml respectively), who undertook a 24-week physical activity programme with blood sampling in the pre-exercise period and at the end of 24-weeks prescribed physical activity. AgilentTM Whole Human Genome Oligo Microarrays were utilised to examine the effects of physical activity on mRNA expression profiles of the Peripheral Blood Mononuclear cells (PBMC) at 2 time points (pre-exercise, and after 24 weeks physical activity. There were 6 individuals per group.
2010-08-03 | E-GEOD-12384 | biostudies-arrayexpress
Project description:Study hypothesis: Evidence suggests that increased physical activity following diagnosis and treatment for colorectal cancer may reduce the risk of disease recurrence after 2.7 years and improve survival by up to 50%. Studies have also shown an association between increased physical activity and other health outcomes for people living with colorectal cancer, for example improved quality of life and improved mental well-being.
Partners may play a role in affecting physical activity behaviour change. The family can be a significant determinant of individual health behaviours. Shared familial environmental factors have been shown to partly account for similarities in physical activity levels amongst family members.
Research has also shown that spousal support is an important factor in smoking cessation, use of health services and weight reduction. Partners may be also a receptive target for physical activity behaviour change. Physical activity consultations may help to increase the physical activity levels and improve the health outcomes of the partners of people living with colorectal cancer as well as the patient.
Primary aim:
To evaluate the feasibility of conducting a randomised controlled trial of physical activity consultations with people living with colorectal cancer and their partners.
Research questions:
1. Is it possible to recruit patients who have completed all surgery and treatment for colorectal cancer in the last 24 months?
2. What would a likely response rate be for a future randomised controlled trial?
3. What are the demographic characteristics of those who are recruited to the study?
4. Can participants be retained in the study for the full 6 months?
5. Is it possible to conduct joint physical activity consultations with people living with colorectal cancer and their partners?
6. Are the outcome measures able to be used and acceptable for use with people living with colorectal cancer and their partners?
7. What would the effect size be for a future power calculation?
Secondary aim:
To evaluate the effects of physical activity consultations on physical activity levels, mental health, quality of life and body composition.
Research questions:
1. What is the effect of physical activity consultations on the physical activity levels of people living with colorectal cancer and their partners?
2. What is the effect of physical activity consultations on depression and anxiety?
3. What is the effect of physical activity consultations on quality of life?
4. What is the effect of physical activity consultations on body composition?
5. Do cancer and other health risk perceptions, intra-couple support and self-efficacy predict any changes in physical activity levels?
Primary outcome(s): Feasibility will be measured by calculating the following rates:
1. Recruitment rate (number of participants randomised/number of eligible participants)
2. Completion rate (number of participants completing baseline and 6 month intervention assessments/number of participants randomised)
3. Adherence rate (number of physical activity consultations and telephone calls conducted/number of physical activity consultations and telephone calls scheduled)
| 2407707 | ecrin-mdr-crc
Project description:Background/Purpose: Prior studies show an independent association between greater physical activity and lower inflammatory markers among adults in the general population, but the impact of physical activity on systemic inflammation in people with rheumatoid arthritis (RA) hasn’t been thoroughly investigated. We therefore aimed to determine whether physical activity behavior associates with differential expression of inflammatory genes in RA. Methods: Data derive from baseline assessments for a prospective observational cohort of patients with RA. At each study visit, we obtained detailed clinical information, collected peripheral whole blood for subsequent RNA sequencing (RNA-seq), and provided participants with actigraphs (GT9X ActiGraph Link device) to objectively measure physical activity by wearing the device at home for 7 consecutive 24-hour periods. Participants were categorized into three physical activity groups based on the highest (“active”), middle (“intermediate”), and lowest (“inactive”) tertiles for percentage of time spent in moderate/vigorous physical activity (metabolic equivalent (MET) level ≥2.00) across the sample. Results: There were 35 patients with complete clinical, actigraphy, and transcriptomic data available for analysis. The participants had a mean age of 56 years, were 90% female, and self-reported the following racial/ethnic identities: 32% white, 11% African American, 8% Asian, 40% Hispanic. Participants spent 48%, 41%, and 12% of time awake in sedentary, light, and moderate physical activity, respectively. None of the participants achieved vigorous activity. The patients in the most active group were younger but did not otherwise significantly differ by clinical and demographic characteristics compared to the other physical activity groups. However, in the differential gene expression analysis comparing the highest versus lowest physical activity tertiles, there were 365 up- and 402 down-regulated genes at an adjusted P value < 0.1. Gene set enrichment analysis revealed that the most physically active patients exhibited downregulation of diverse innate and adaptive immune signaling pathways. Assessment of upstream cytokine activation states demonstrated inhibition of type I, II and III interferons and activation of EPO in the highest physical activity tertile patients. Conclusion: Among a racially and ethnically diverse RA cohort, participants who spent more time in moderately intense physical activity had down-regulation of genes involved in both innate and adaptive immune signaling compared to those who were more sedentary. These findings provide mechanistic evidence to support a disease-modifying effect of physical activity in RA.
2021-09-01 | GSE179302 | GEO
Project description:This study is a two-arm trial, comparing the effects of a virtual 12-week group-based exercise intervention vs. control group on physical activity, physical fitness, and quality of life in colorectal cancer survivors, and explore multi-level determinants of physical activity maintenance, 6-months after intervention completion.
| 2292592 | ecrin-mdr-crc
Project description:Objective: To evaluate gene expression profiles in multiple sclerosis (MS) patients who improved their fatigue status after a program of physical exercise and to compare them with healthy controls (HC). Methods: A prospective longitudinal study was conducted. Gene expression in whole blood was profiled at baseline in 7 healthy controls and also in 7 fatigued-MS patients. Patients underwent a physical exercise program for 6 months, and their fatigue status and gene expression profiles were again analyzed at the end of this program. Results: MS patients showed a significant activation of genes participating in the systemic interferon response in comparison with healthy controls. Fatigue improved at the end of the physical activity program, and, in parallel, systemic activation of interferon related genes disappeared. Conclusions: Fatigue improvement following an exercise program is associated to down modulation of interferon activity at the systemic level in MS patients. Our results provide a biological basis for the observed benefit of physical exercise in MS.
2015-09-27 | E-MTAB-2973 | biostudies-arrayexpress
Project description:Physical Activity Moderates Inflammatory Gene Expression in Rheumatoid Arthritis
| PRJNA743095 | ENA