Project description:To identify candidate downstream mRNA target for hsa-miR-130b miR-130b or empty vector control was overexpressed in PLC8024 CD133- HCC cells using lentivirus
Project description:Proteome data derive from three different types of cells （uninfected gADSCs, gADSCs infected with the empty lentiviral vector, and gADSCs infected with the miR-204-5p lentiviral vector).
Project description:In order to examine the consequences of human miR-34a induction on the transcriptome, HCT116 cells (a colon cancer cell line) were infected with a retrovirus that produces miR-34a. Gene expression profiles were then monitored using Affymetrix microarrays. Affymetrix microarrays were used to examine the transcriptomes of HCT116 cells infected with an empty retroviral vector (pMSCV-PIG) or a retroviral vector that expresses human miR-34a. Keywords: comparison of cells with or without enforced miR-34a expression
Project description:Purpose: identify genes regulated by expression of miR-31 in primary mouse CD8 T-cells by exogenously expressing pre-miR-31 from the Plko.3g lentiviral vector. Cells infected with empty Plko.3g vectors were used as controls for infection.
Project description:Interactome of OCIAD1-FLAG was studied. TMT-based relative quantification of protein levels was performed in eluates from mitochondria of HEK293 cells that expressed OCIAD1-FLAG vs empty vector and from mitochondria of OCIAD1-KO cells that expressed OCIAD1-FLAG vs empty vector.
Project description:To identify targets of miR-203a we coupled RNA-seq and AGO2-RNA immunoprecipitation (RIP-seq) in MCF10A cells expressing a control pre-miR-203a or control, empty vector
